Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Digital PCR: A partitioning-based application for detection and surveillance of SARS-CoV-2 from sewage samples.- Digital PCR: A tool to authenticate herbal products and spice.- Emulsion Polymerase Chain Reaction coupled with Denaturant Gradient Gel Electrophoresis for microbial diversity studies.- Real-Time PCR High-Resolution Melting Assays for the Detection of Foodborne Pathogens.- High-Throughput real-time qPCR and High-Resolution Melting (HRM) assay for fungal detection in plant matrices.- Multiplex real-time PCR for the detection of Shiga Toxin-producing Escherichia coli in foods.- DNA isolation from cocoa-derived products and cocoa authentication by TaqMan real-time PCR.- Quantitative real-time PCR for the detection of allergenic species in foods.- Accurate absolute quantification of bacterial populations in mixed cultures by qPCR.- Real-time PCR method for assessment of ParA-mediated recombination efficiency in minicircle production.- Gene expression quantification from pathogenic bacterial biofilms by quantitative PCR.- A real-time quantitative PCR protocol for the quantification of plasmid copy number in Lactococcus lactis.- Improved PCR by the use of disruptors, a new class of oligonucleotide reagents.- Mitochondrial DNA D-loop amplification and sequencing for species differentiation in milk.- Long-range Polymerase Chain Reaction.- Megaprimer-based PCR to synthesise fusion genes for cloning.- Bacteria and Yeast ColonyPCR.- Inverse PCR for site-directed mutagenesis.- Optimized design of degenerate primers for PCR based on DNA or protein sequence comparisons.