Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts

Part 1 : Isolation and culture of skin cells.- Chap 1: Isolation and Culture of Human Keratinocytes.- Chap 2 : Promotion of Human Epidermal Keratinocyte Expansion in Feeder Cell Co-culture.- Chap 3 : Isolation and Culture of Epidermal Melanocytes.- Chap 4: Long-term Expansion of Mouse Primary Epidermal Keratinocytes Using a TGF-beta Signaling Inhibitor.- Chap 5 : Isolation and Culture of Hair Follicle Dermal Sheath Mesenchymal Stromal Cells.- Chap 6 : Isolation and Culture of Human Dermal Fibroblasts.- Chap 7: Isolation and Culture of Human Dermal Microvascular Endothelial Cells.- Chap 8: Isolation of Stromal Vascular Fraction by Fractionation of Adipose Tissue.- Part 2 : Tissue engineering skin and engineered skin models.- Chap 9 : Engineering a Multi-Layered Skin Equivalent: the Importance of Endogenous Extracellular Matrix Maturation to Provide Robustness and Reproducibility.- Chap 10 : Three-dimensional Epidermal Model from Human Hair follicle-derived Keratinocytes.- Chap 11 : Fabrication of a Co-Culture System with Human Sweat Gland Derived Cells and Peripheral Nerve Cells.- Chap 12 : Engineering a Multilayered Skin Substitute with Keratinocytes, Fibroblasts, Adipose Derived Stem Cells and Adipocytes.- Chap 13 : Skin Tissue Engineering Fabrication of Chimeric Hair Follicles for Skin Tissue Engineering.- Chap 14 : Isolation and Culture of Epidermolysis Bullosa Cells and Organotypic Skin Models.- Part 3 : In vitro and vivo models testing skin, skin cells, and tissue engineered skin.- Chap 15 : Effects of the Extracellular Matrix on the Proteome of Primary Skin Fibroblasts.- Chap 16 : Standard Preparation Protocol of Human Skin Samples for Transmission Electron Microscopy.- Chap 17 : Methods for Assessing Scaffold Vascualarization In Vivo.- Chap 18 : Human Reconstructed Skin in a Mouse Model.- Chap 19 : Pig Model to Test Tissue Engineered Skin.- Chap 20 : Transplantation of Autologous Dermo-Epidermal Skin Substitutes in a Pig Model.