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Authoritative and cutting-edge, Recombinant Protein Production in Yeast: Methods and Protocols aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications.
Authoritative and cutting-edge, Recombinant Protein Production in Yeast: Methods and Protocols aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications.
Include cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Established and Upcoming Yeast Expression Systems.- Detection and Elimination of Cellular Bottlenecks in Protein Producing Yeasts.- Pichia pastoris Promoters.- Selection of the Optimal Yeast Host for the Synthesis Recombinant Enzymes.- Screening and Selection of Production Strains: Secretory Protein Expression and Analysis in Hansenula polymorpha.- Selection of Heterologous Protein Producing Strains in Yarrowia lipolytica.- High-throughput Screening and Selection of Pichia pastoris Strains.- Analyzing Recombinant Protein Production in Pichia pastoris with Targeted Proteomics Roslyn M. Bill, Annegret Ulke.- CRISPR/Cas9-mediated Homology Directed Genome Editing in Pichia pastori.- Tailoring N-glycan Biosynthesis for Production of Therapeutic Proteins in Saccharomyces cerevisiae.- Improving the Production of Cofactor Containing Proteins: Production of Human Hemoglobin in Yeast.- Membrane Protein Production in Yeast: Modification of Yeast Membranes for Human Membrane Protein Production.- Yeast Surface Display and Cell Sorting of Antigen-binding Fc Fragments.- Guidelines for Small-scale Production and Purification of Hepatitis B surface Antigen Virus-like Particles from Recombinant Pichia pastoris.- Efficient Development of a Mixed Feed Process for Pichia pastoris.- Primary Recovery of Yeast Culture Supernatant for Recombinant Protein Purification.- Purification of Recombinant glyco-proteins from Pichia pastoris Culture Supernatants.- LC-MS analysis of (glyco)-proteins of Pichia pastoris.- Assessing the Quality of Recombinant Products Made in Yeast.- Quantification of Recombinant Products In Yeast.
| Erscheinungsjahr: | 2019 |
|---|---|
| Fachbereich: | Gentechnologie |
| Genre: | Biologie |
| Rubrik: | Naturwissenschaften & Technik |
| Medium: | Buch |
| Seiten: | 444 |
| Reihe: | Methods in Molecular Biology |
| Inhalt: |
xii
431 S. 14 s/w Illustr. 46 farbige Illustr. 431 p. 60 illus. 46 illus. in color. |
| ISBN-13: | 9781493990238 |
| ISBN-10: | 1493990233 |
| Sprache: | Englisch |
| Herstellernummer: | 978-1-4939-9023-8 |
| Ausstattung / Beilage: | HC runder Rücken kaschiert |
| Einband: | Gebunden |
| Redaktion: |
Mattanovich, Diethard
Gasser, Brigitte |
| Herausgeber: | Brigitte Gasser/Diethard Mattanovich |
| Auflage: | 1st ed. 2019 |
| Hersteller: |
Springer New York
Springer US, New York, N.Y. Methods in Molecular Biology |
| Maße: | 260 x 183 x 30 mm |
| Von/Mit: | Diethard Mattanovich (u. a.) |
| Erscheinungsdatum: | 09.02.2019 |
| Gewicht: | 1,027 kg |
Include cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Established and Upcoming Yeast Expression Systems.- Detection and Elimination of Cellular Bottlenecks in Protein Producing Yeasts.- Pichia pastoris Promoters.- Selection of the Optimal Yeast Host for the Synthesis Recombinant Enzymes.- Screening and Selection of Production Strains: Secretory Protein Expression and Analysis in Hansenula polymorpha.- Selection of Heterologous Protein Producing Strains in Yarrowia lipolytica.- High-throughput Screening and Selection of Pichia pastoris Strains.- Analyzing Recombinant Protein Production in Pichia pastoris with Targeted Proteomics Roslyn M. Bill, Annegret Ulke.- CRISPR/Cas9-mediated Homology Directed Genome Editing in Pichia pastori.- Tailoring N-glycan Biosynthesis for Production of Therapeutic Proteins in Saccharomyces cerevisiae.- Improving the Production of Cofactor Containing Proteins: Production of Human Hemoglobin in Yeast.- Membrane Protein Production in Yeast: Modification of Yeast Membranes for Human Membrane Protein Production.- Yeast Surface Display and Cell Sorting of Antigen-binding Fc Fragments.- Guidelines for Small-scale Production and Purification of Hepatitis B surface Antigen Virus-like Particles from Recombinant Pichia pastoris.- Efficient Development of a Mixed Feed Process for Pichia pastoris.- Primary Recovery of Yeast Culture Supernatant for Recombinant Protein Purification.- Purification of Recombinant glyco-proteins from Pichia pastoris Culture Supernatants.- LC-MS analysis of (glyco)-proteins of Pichia pastoris.- Assessing the Quality of Recombinant Products Made in Yeast.- Quantification of Recombinant Products In Yeast.
| Erscheinungsjahr: | 2019 |
|---|---|
| Fachbereich: | Gentechnologie |
| Genre: | Biologie |
| Rubrik: | Naturwissenschaften & Technik |
| Medium: | Buch |
| Seiten: | 444 |
| Reihe: | Methods in Molecular Biology |
| Inhalt: |
xii
431 S. 14 s/w Illustr. 46 farbige Illustr. 431 p. 60 illus. 46 illus. in color. |
| ISBN-13: | 9781493990238 |
| ISBN-10: | 1493990233 |
| Sprache: | Englisch |
| Herstellernummer: | 978-1-4939-9023-8 |
| Ausstattung / Beilage: | HC runder Rücken kaschiert |
| Einband: | Gebunden |
| Redaktion: |
Mattanovich, Diethard
Gasser, Brigitte |
| Herausgeber: | Brigitte Gasser/Diethard Mattanovich |
| Auflage: | 1st ed. 2019 |
| Hersteller: |
Springer New York
Springer US, New York, N.Y. Methods in Molecular Biology |
| Maße: | 260 x 183 x 30 mm |
| Von/Mit: | Diethard Mattanovich (u. a.) |
| Erscheinungsdatum: | 09.02.2019 |
| Gewicht: | 1,027 kg |
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