Include cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Established and Upcoming Yeast Expression Systems.- Detection and Elimination of Cellular Bottlenecks in Protein Producing Yeasts.- Pichia pastoris Promoters.- Selection of the Optimal Yeast Host for the Synthesis Recombinant Enzymes.- Screening and Selection of Production Strains: Secretory Protein Expression and Analysis in Hansenula polymorpha.- Selection of Heterologous Protein Producing Strains in Yarrowia lipolytica.- High-throughput Screening and Selection of Pichia pastoris Strains.- Analyzing Recombinant Protein Production in Pichia pastoris with Targeted Proteomics Roslyn M. Bill, Annegret Ulke.- CRISPR/Cas9-mediated Homology Directed Genome Editing in Pichia pastori.- Tailoring N-glycan Biosynthesis for Production of Therapeutic Proteins in Saccharomyces cerevisiae.- Improving the Production of Cofactor Containing Proteins: Production of Human Hemoglobin in Yeast.- Membrane Protein Production in Yeast: Modification of Yeast Membranes for Human Membrane Protein Production.- Yeast Surface Display and Cell Sorting of Antigen-binding Fc Fragments.- Guidelines for Small-scale Production and Purification of Hepatitis B surface Antigen Virus-like Particles from Recombinant Pichia pastoris.- Efficient Development of a Mixed Feed Process for Pichia pastoris.- Primary Recovery of Yeast Culture Supernatant for Recombinant Protein Purification.- Purification of Recombinant glyco-proteins from Pichia pastoris Culture Supernatants.- LC-MS analysis of (glyco)-proteins of Pichia pastoris.- Assessing the Quality of Recombinant Products Made in Yeast.- Quantification of Recombinant Products In Yeast.