Provides a comprehensive set of methods used in fungal molecular and cell biology written by leading researchers in the field

Presents the most popular methods alongside highly advanced technologies

Methods cover all types of molecular and cellular research from single cell analyses to high throughput methods

Methods will prove invaluable to novice as well as experienced researchers

Methods have been proven effective for a wide variety of fungal species

Includes supplementary material: [...]

Fungal Transformation and Gene Knockout.- Protoplast Transformation of Filamentous Fungi.- Electroporation and Agrobacterium-Mediated Spore Transformation.- High-Throughput Construction of Gene Deletion Cassettes for Generation of Neurospora crassa Knockout Strains.- Development of Impala-Based Transposon Systems for Gene Tagging in Filamentous Fungi.- DelsGate: A Robust and Rapid Method for Gene Deletion.- Gene Silencing for Functional Analysis: Assessing RNAi as a Tool for Manipulation of Gene Expression.- Detection and Quantification of Fungi.- Analysis of Fungal Gene Expression by Real Time Quantitative PCR.- Identification of Differentially Expressed Fungal Genes In Planta by Suppression Subtraction Hybridization.- Quantification of Fungal Infection of Leaves with Digital Images and Scion Image Software.- Expression Profiling of Fungal Genes During Arbuscular Mycorrhiza Symbiosis Establishment Using Direct Fluorescent In Situ RT-PCR.- Application of Laser Microdissection to Study Plant-Fungal Pathogen Interactions.- Multiplex Gene Expression Analysis by TRAC in Fungal Cultures.- Amplification of Fungal Genomes Using Multiple Displacement Amplification.- Microscopy and Protein Analysis.- Biochemical Methods Used to Study the Gene Expression and Protein Complexes in the Filamentous Fungus Neurospora crassa.- Measuring Protein Kinase and Sugar Kinase Activity in Plant Pathogenic Fusarium Species.- A Detailed Protocol for Chromatin Immunoprecipitation in the Yeast Saccharomyces cerevisiae.- A Method to Visualize the Actin and Microtubule Cytoskeleton by Indirect Immunofluorescence.- Fluorescence In Situ Hybridization for Molecular Cytogenetic Analysis in Filamentous Fungi.- Live-Cell Imaging of Microtubule Dynamics in Hyphae of Neurospora crassa.- Methods to DetectApoptotic-Like Cell Death in Filamentous Fungi.- Evaluation of Antifungal Susceptibility Using Flow Cytometry.- Preparation of Fungi for Ultrastructural Investigations and Immunogoldlabelling.- Split-EGFP Screens for the Detection and Localisation of Protein-Protein Interactions in Living Yeast Cells.