Sophia Y. Lunt: Metabolic Signaling | Buch

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Cover: 9781493987689 | Metabolic Signaling | Methods and Protocols | Sophia Y. Lunt (u. a.)

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Sprache: Englisch

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Beschreibung

This book provides protocols to quantify metabolism, to identify metabolic crosstalk, and to setup and develop tools and models to gain insight into metabolic signaling using experimental and computational approaches. Chapters detail protocols to quantify metabolism, identify metabolic crosstalk, and develop tools and models to gain a systems-level insight into metabolic signaling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Metabolic Signaling: Methods and Protocols aims to provide researchers with methods to study, perturb, and functionally interpret metabolism and metabolic signaling from the sub-cellular to the whole-body level.

Zusammenfassung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Inhaltsverzeichnis

A Protocol to Compare Methods for Untargeted Metabolomics.- High Throughput Measure of Bioactive Lipids Using Non-targeted Mass Spectrometry.- Measuring the Nutrient Metabolism of Adherent Cells in Culture.- 13C Tracer Analysis and Metabolomics in 3D Cultured Cancer Cells.- Measuring In Vivo Tissue Metabolism using 13C Glucose Infusions in Mice.- Measuring Human Lipid Metabolism using Deuterium Labeling: In vivo and in vitro.- Measuring Rates of ATP Synthesis.- Direct Estimation of Metabolic Flux by Heavy Isotope Labeling Simultaneous with Pathway Inhibition: Metabolic Flux Inhibition Assay.- Measuring Glycolytic and Mitochondrial Fluxes in Endothelial Cells using Radioactive Tracers.- Determining Compartment Specific Metabolic Fluxes.- Determining the Impact of Metabolic Nutrients on Autophagy.- Measuring the Activation of Cell Death Pathways upon Inhibition of Metabolism.- Determining Macrophage Polarization upon Metabolic Perturbation.- Assessing the Impact of the Nutrient Microenvironment on The Metabolism of Effector CD8+ T Cells.- Development of Patient-derived Tumor Xenograft Models.- Imaging Glioma Progression by Intravital Microscopy.- Lipectomizing Mice for Applications in Metabolism.- Quantitative Multiplex Immunoassay for Profiling Bone Turnover Biomarkers in Human Bone Tissue Culture Supernatants.- Determining the Intracellular Organization of Organelles.- The Fundamentals of Constructing and Interpreting Heat Maps.

Details

Erscheinungsjahr:	2018
Fachbereich:	Allgemeines
Genre:	Biologie
Rubrik:	Naturwissenschaften & Technik
Thema:	Lexika
Medium:	Buch
Seiten:	308
Reihe:	Methods in Molecular Biology
Inhalt:	xii 294 S. 14 s/w Illustr. 53 farbige Illustr. 294 p. 67 illus. 53 illus. in color.
ISBN-13:	9781493987689
ISBN-10:	1493987682
Sprache:	Englisch
Herstellernummer:	978-1-4939-8768-9
Ausstattung / Beilage:	HC runder Rücken kaschiert
Einband:	Gebunden
Redaktion:	Lunt, Sophia Y. Fendt, Sarah-Maria
Herausgeber:	Sarah-Maria Fendt/Sophia Y Lunt
Auflage:	1st ed. 2019
Hersteller:	Springer US Springer New York Methods in Molecular Biology
Maße:	260 x 183 x 22 mm
Von/Mit:	Sophia Y. Lunt (u. a.)
Erscheinungsdatum:	13.10.2018
Gewicht:	0,849 kg