Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: [...]

Production of Eukaryotic Cell-Free Lysate from Leishmania tarentolae.- Bioinformatics Analysis and Optimization of Cell-Free Protein Expression.- A Cell-Free Expression Screen to Identify Fusion Tags for Improved Protein Expression.- One-Pot, Microscale Cell-Free Enzyme Expression and Screening.- Cell-Free Translation of Biofuels Enzymes.- Cloning-Independent Expression and Screening of Enzymes using Cell-Free Protein Synthesis Systems.- High Level Cell-Free Production of Membrane Proteins with Nanodiscs.- Cell Free Protein Based Enzyme Discovery and Protein-Ligand Interaction Study.- Human Cell Extract-Derived Cell-Free Systems for Virus Synthesis.- Cell-Free Protein Synthesis in Microfluidic 96-Well Plates.- Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein Synthesis.- Site-Specific Incorporation of Unnatural Amino Acids into Proteins by Cell-Free Protein Synthesis.- In vitro Translation of Papillomavirus Authentic and Codon-Modified L1 Capsid Gene mRNAs in Mouse Keratinocyte Cell-Free Lysate.- An Optimized Yeast Cell-Free Lysate System for In vitro Translation of Human Virus mRNA.- In vitro Translation Based Protein Kinase Substrate Identification.- Preparation of Protein Arrays using Cell-Free Protein Expression.- Post-Transcriptional Control of Protein Synthesis in Drosophila S2 Cell-Free System.- Cell-Free Membrane Protein Expression.- The PURE System for Protein Production.- A Cell-Free Protein Synthesis System from Insect Cells.- A Cell-Free Expression Platform for Production of Protein Microarrays.