Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Overview of Mitochondrial Bioenergetics.- Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells.- High-Resolution FluoRespirometry and OXPHOS Protocols for Human Cells, Permeabilized Fibres from Small Biopsies of Muscle and Isolated Mitochondria.- High-Throughput Analysis of Mitochondrial Oxygen Consumption.- Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices.- Mitochondrial Membrane Potential (¿¿) Fluctuations Associated with the Metabolic States of Mitochondria.- Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells.- Comparison of Mitochondrial Incubation Media for Measurement of Respiration and Hydrogen Peroxide Production.- Measurement of Proton Leak in Isolated Mitochondria.- Imaging Mitochondrial Calcium Fluxes with Fluorescent Probes and Single or Two Photon Confocal Microscopy.- Mitochondrial Permeability Transition Pore and Calcium Handling.- Redox Equivalents and Mitochondrial Bioenergetics.- Mitochondrial Bioenergetics by 13 C-NMR Isotopomer Analysis.- Computational Modeling of Mitochondrial Function from a Systems Biology Perspective.- Monitoring the Mitochondrial Dynamics in Mammalian Cells.- Plate-Based Measurement of Superoxide and Hydrogen Peroxide Production by Isolated Mitochondria.- Plate-Based Measurement of Respiration by Isolated Mitochondria.- Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian Cells.- The Importance of Calcium Ions for Determining Mitochondrial Glycerol-3-Phosphate Dehydrogenase Activity When Measuring Uncoupling Protein 1 (UCP1) Function in Mitochondria Isolated from Brown Adipose Tissue.- Isolation and Analysis of Mitochondrial Small RNAs from Rat Liver Tissue and HepG2 Cells.- Imaging of Mitochondrial pH Using SNARF-1.- Relation between Mitochondrial Membrane Potential and ROS Formation.- Assessing Spatio-Temporal and Functional Organization of Mitochondrial Networks.- Measurement of Mitochondrial ROS Formation.