Includes cutting-edge methods and protocols involving insoluble proteins

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: [...]

General Introduction: Recombinant Protein Production and Purification of Insoluble Proteins.- Overcoming the Solubility Problem in
E. coli
: Available Approaches for Recombinant Protein Production.- Optimization of Culture Parameters and Novel Strategies to Improve Protein Solubility.- Cleavable Self-Aggregating Tags (cSAT) for Protein Expression and Purification.- Beyond the Cytoplasm of
Escherichia coli
: Localizing Recombinant Proteins Where You Want Them.- Characterization of Amyloid-Like Properties in Bacterial Intracellular Aggregates.- Co-Translational Stabilization of Insoluble Proteins in Cell-Free Expression Systems.- Functional Expression of Plant Membrane Proteins in
Lactococcus lactis.
- High Cell-Density Expression System: Yeast Cells in a Phalanx Efficiently Produce a Certain Range of "Difficult-to-Express" Secretory Recombinant Proteins.- Insect Cells-Baculovirus System for the Production of Difficult to Express Proteins.- Transient Expression in HEK 293 Cells: An Alternative to E. coli for the Production of Secreted and Intracellular Mammalian Proteins.- Recombinant Glycoprotein Production in Human Cell Lines.- Soluble Recombinant Protein Production in
Pseudoaltermonas haloplanktis
TAC125.- A Screening Methodology for Purifying Proteins with Aggregation Problems.- Solubilization and Refolding of Inclusion Body Proteins.- Bacterial Inclusion Body Purification.- Characterization of Intracellular Aggresomes by Fluorescent Microscopy.- Dialysis: A Characterization Method of Aggregation Tendency.- Applications of Mass Spectrometry to the Study of Protein Aggregation.- Insoluble Protein Assemblies Characterized by Fourier Transform Infrared Spectroscopy.- Insoluble Protein Characterization by Circular Dichroism (CD) Spectroscopy and Nuclear Magnetic Resonance (NMR).- Methods for Characterization of Protein Aggregates.- Predicting the Solubility of Recombinant Proteins in
Escherichia coli
.- Insoluble ProteinApplications: The Use of Bacterial Inclusion Bodies as Biocatalysts.