Details many of the methods currently used to study RNA silencing in relation to viral infections in plants

Features step-by-step, easy to follow protocols from an international assortment of laboratories

Includes key tips and expert implementation advice to ensure successful results

An Historical Overview of RNAi in Plants.- RNA Silencing and Antiviral Defense in Plants.- Biological Chemistry of Virus-encoded Suppressors of RNA Silencing: An Overview.- Virus-Induced Gene Silencing Using Begomovirus Satellite Molecules.- Strategies for Viral Cross Protection in Plants.- Tobacco Rattle Virus (TRV)-Based Virus-Induced Gene Silencing.- Virus-Induced Gene Silencing in Hexaploid Wheat Using Barley Stripe Mosaic Virus Vectors.- Characterization of Plant Virus-Encoded Gene Silencing Suppressors.- Generation of Plant Small RNA cDNA Libraries for High-Throughput Sequencing.- Modelling Antiviral Resistance in Plants.- Isolation and Detection of Small RNAs from Plant Tissues.- Isolation and Analysis of Small RNAs from Virus-Infected Plants.- Use of Hairpin RNA Constructs for Engineering Plant Virus Resistance.- Artificial MicroRNAs for Plant Virus Resistance.- Isolation and Biochemical Analysis of Plant Small RNAs.- Isolation and Analysis of Plant RNA-Dependent RNA Polymerases.- Plant Viroids: Isolation, Characterization/Detection, and Analysis.- Structural and Functional Analysis of CMV Satellite RNAs in RNA Silencing.- Virus-Induced Gene Silencing in Soybean.- Chimeric, Infectious, and Stable Virus Transcripts to Study RNA Silencing in 'Dark Green' Islands.- Rolling Circle Amplification-Mediated Hairpin RNA (RMHR) Library Construction in Plants.- The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing.- Identification of Small Molecule Inhibitors of Tomato Bushy Stunt Virus (TBSV) Replication.- Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections.