Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: [...]

Use of IMGT® Databases and Tools for Antibody Engineering and Humanization.- Computer-Assisted Modeling of Antibody Variable Domains.- Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells.- Human in Cell scFv Library from Infiltrating B Cell.- Construction of Human Naive Antibody Gene Libraries.- Synthetic Customized scFv Libraries.- Selection of Stable scFv Antibodies by Phage Display.- Generation of Single Domain Antibody Fragments Derived from Camelids and Generation of Manifold Constructs.- Generation and Isolation of Target Specific Single Domain Antibodies from Shark Immune Repertoires.-Generation of Human Single Domain Antibody Repertoires by Kunkel Mutagenesis.- Phage Display and Selections on Purified Antigens.- Phage Display and Selections on Cells.- Humanization by CDR Grafting and Specificity-Determining Residue Grafting.-Humanization by Guided Selections.- Selection of Antibody Fragments by Yeast Display.- Evolution of Antibodies in vitro by Ribosome Display.- Mammalian Cell Display of Full Length IgG.- Production of Antibody Fragments in Escherichia coli.-Production of Antibody Derivatives in the Methylotrophic Yeast Pichia pastoris.- Monoclonal Antibody Expression in Mammalian Cells.- Production of Recombinant Antibodies in Drosophila melanogaster S2 Cells.- Production of Antibody Fragments Using the Baculovirus-Insect Cell System.- Transient and Stable Expression of Antibodies in Nicotiana Species.- Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance.- Affinity Determination of Biotinylated Antibodies by Flow Cytometry.- Affinity Maturation of Antibodies: Optimized Methods to Generate High Quality scFv Libraries and Isolate IgG Candidates by High Throughput Screening.- Affinity Maturation by Semi Rational Approaches.- Molecular Scanning Mutagenesis: Combining Ribosome Display, Random Mutagenesis and Bioinformatic Analysis for Protein Engineering.- Fucose-Targeted Glyco-Engineering of PharmaceuticalCell Lines.- Fc engineering: Design, expression and functional characterization of antibody variants with improved effector function.- Fc engineering: Serum half life modulation through FcRn binding.- Monoclonal Antibody Lead Characterization - In vitro and in vivo methods.- Production, generation and characterization of scFv TNF ligand fusion proteins.- Antibody-IL-2 fusion proteins for tumor targeting.- Recombinant immunotoxins containing low endotoxins for clinical and animal studies.- Chimeric antigen receptors for T-cell based therapy.- Selection and Use of Intracellular Antibodies (Intrabodies).- Radiolabeled Antibodies for Imaging and Therapy.- Design and Production of Multimeric Antibody Fragments, Focused on Diabodies with Enhanced Clinical Efficacy.- Production of Bispecific Antibodies: Diabodies and Tandem scFv.