Ali A. Abdul-Sater: The Inflammasome

Cover: 9781071621462 | The Inflammasome | Methods and Protocols | Ali A. Abdul-Sater | Buch

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Beschreibung

This volume provides inflammasome instructions and tips to study different aspects of inflammasome biology. Chapters guide readers through how to assess canonical and non-canonical inflammasome activation, in vitro immune and non-immune cells, whole blood, biochemical assays to evaluate ASC oligomerization, gasdermin D cleavage, and mapping phosphorylated residues of NLRP3. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, The Inflammasome: Methods and Protocols aims to provide protocols that will serve as tools for current and aspiring researchers interested in studying inflammasomes.

Zusammenfassung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Inhaltsverzeichnis

Assessment of ASC Oligomerization by Flow Cytometry.- In-vitro Assays to Study Inflammasome Activation in Primary Macrophages.- Measuring Non-Canonical Inflammasome Activation In Neutrophils.- Gasdermin D Cleavage Assay Following Inflammasome Activation.- Activation of the Non-Canonical Inflammasome In Mouse And Human Cells.- Measurement of Inflammasome-Induced Mitochondrial Dysfunction By Flow Cytometry.- Detection of ASC Oligomerization by Western Blotting.- Reconstitution System of NLRP3 Inflammasome in HEK293T Cells.- Intracellular Potassium Ions Measurements by Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES).- NLRP3 Phospho-Residue Mapping by Phospho Dot-Blots.- Analysis of Activity and Expression of the NLRP3, AIM2 and NLRC4 Inflammasome in Whole Blood.- Measurement of Cytosolic Mitochondrial DNA after NLRP3 Inflammasome Activation.- Inflammasome-derived Exosomes Isolation and Imaging by Transmission Electron Microscopy.- In Vitro and In Vivo Model for Sepsis through Non-Canonical NLRP3 inflammasome Activation.- Inflammasome Activation in Gingival Epithelial Cells.- Imaging of Inflammasome Speck Formation in Living Cells.

Erscheinungsjahr:	2023
Fachbereich:	Therapie
Genre:	Medizin
Rubrik:	Wissenschaften
Medium:	Taschenbuch
Reihe:	Methods in Molecular Biology
Inhalt:	x 180 S. 16 s/w Illustr. 14 farbige Illustr. 180 p. 30 illus. 14 illus. in color.
ISBN-13:	9781071621462
ISBN-10:	1071621467
Sprache:	Englisch
Ausstattung / Beilage:	Paperback
Einband:	Kartoniert / Broschiert
Redaktion:	Abdul-Sater, Ali A.
Herausgeber:	Ali A Abdul-Sater
Auflage:	1st ed. 2022
Hersteller:	Springer US Springer US, New York, N.Y. Methods in Molecular Biology
Maße:	254 x 178 x 11 mm
Von/Mit:	Ali A. Abdul-Sater
Erscheinungsdatum:	27.02.2023
Gewicht:	0,373 kg

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