Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: [...]

Part I. mRNA Synthesis, Purification, and Transfer 1. Cell Engineering with Synthetic Messenger RNA Peter M. Rabinovich and Sherman M. Weissman 2. In vitro Transcription of Long RNA Containing Modified Nucleosides Norbert Pardi, Hiromi Muramatsu, Drew Weissman, and Katalin Karikó 3. HPLC Purification of in vitro Transcribed Long RNA Drew Weissman, Norbert Pardi, Hiro Muramatsu, and Katalin Karikó 4. Synthetic mRNAs with Superior Translation and Stability Properties Ewa Grudzien-Nogalska, Joanna Kowalska, Wei Su, Andreas N. Kuhn, Sergey V. Slepenkov, Edward Darzynkiewicz, Ugur Sahin, Jacek Jemielity, and Robert E. Rhoads 5. Non-Viral, Cationic Lipid-Mediated Delivery of mRNA James G. Hecker 6. Light-Induced mRNA Transfection Sigurd Leinæs Bøe and Eivind Hovig 7. Engineering B Cells with mRNA Jaewoo Lee, David Boczkowski, and Smita Nair 8. Programming Human Dendritic Cells with mRNA Jaewoo Lee, David Boczkowski, and Smita Nair 9. Large Volume Flow Electroporation of mRNA: Clinical Scale Process Linhong Li, Cornell Allen, Rama Shivakumar, and Madhusudan V. Peshwa 10. Retrovirus-Based mRNA Transfer for Transient Cell Manipulation Melanie Galla, Axel Schambach, and Christopher Baum 11. mRNA Delivery to Human Dendritic Cells by Recombinant Yeast and Activation of Antigen-Specific Memory T Cells Frank Breinig, Tanja Breinig, and Manfred J. Schmitt Part 2. Modulation and monitoring of cell metabolism 12. Non-Viral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy Tobias Riet, Astrid Holzinger, Jan Dörrie, Niels Schaft, Gerold Schuler, and Hinrich Abken 13. Natural Killer Cell Reprogramming with Chimeric Immune Receptors Noriko Shimasaki and Dario Campana 14. Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA Peizhe Wang and Jie Na 15. Anti-Tumor Vaccination with Synthetic mRNA: Strategies for in vitro and in vivo Preclinical Studies Mustafa Diken, Sebastian Kreiter, Abderraouf Selmi, Özlem Türeci, and Ugur Sahin 16. Mannosylated and Histidylated LPR Technology for Vaccination with Tumor Antigen mRNA Chantal Pichon and Patrick Midoux 17. Analysis of Surviving-Specific T Cells in Breast Cancer Patients Using Human DCs Engineered with Survivin mRNA Özcan Met and Inge Marie Svane 18. mRNA electroporation as a Tool for Immunomonitoring Nathalie Cools, Kirsten Van Camp, Viggo Van Tendeloo, and Zwi Berneman 19. mRNA PCR-Based Epitope Chase Method Jean-Daniel Doucet, Dominique Gauchat, and Réjean Lapointe Part 2. Modulation and monitoring of cell metabolism 12. Non-Viral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy Tobias Riet, Astrid Holzinger, Jan Dörrie, Niels Schaft, Gerold Schuler, and Hinrich Abken 13. Natural Killer Cell Reprogramming with Chimeric Immune Receptors Noriko Shimasaki and Dario Campana 14. Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA Peizhe Wang and Jie Na 15. Anti-Tumor Vaccination with Synthetic mRNA: Strategies for in vitro and in vivo Preclinical Studies Mustafa Diken, Sebastian Kreiter, Abderraouf Selmi, Özlem Türeci, and Ugur Sahin 16. Mannosylated and Histidylated LPR Technology for Vaccination with Tumor Antigen mRNA Chantal Pichon and Patrick Midoux 17. Analysis of Surviving-Specific T Cells in Breast Cancer Patients Using Human DCs Engineered with Survivin mRNA Özcan Met and Inge Marie Svane 18. mRNA electroporation as a Tool for Immunomonitoring Nathalie Cools, Kirsten Van Camp, Viggo Van Tendeloo, and Zwi Berneman 19. mRNA PCR-Based Epitope Chase Method Jean-Daniel Doucet, Dominique Gauchat, and Réjean Lapointe