Dr. Ratan K. Choudhary is an Assistant Professor at the College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India. He holds a master's degree in veterinary sciences and a doctoral degree in animal science with a specialization in bovine mammary stem cells and their characterization. His research chiefly focuses on the development of stem cell therapy for the dry period and mastitis in dairy animals. He worked at the United States Department of Agriculture (USDA) for six years (2007-2012), followed by two post-doctoral trainings at the University of Kentucky (2012-2013) and University of Vermont (2018-2020), USA. He received many awards and fellowships during his Ph.D. including the Dean's Fellowship, R. F. Davis Scholarship and Jacob K. Goldhaber Travel Award, Beltsville Agricultural Research Center, USDA poster awards, first prize winner of the National Milk Producers Federation, Graduate Student Paper Presentation Award in Dairy Production, and Novus International Inc. Travel Award. He is currently serving as a reviewer of the international journal,s including Journal of Mammary Gland Biology and Neoplasia, Scientific Reports, World Journal Stem Cells, Journal of Dairy Research, PLOS One, and Stem Cells Reviews and Report. He has published more than 40 research articles in peer-reviewed international journals, attended/presented research papers at more than 30 conferences, and authored one book. He is a member of various international scientific societies and organizations, e.g., the American Dairy Science Association, the European Association for Animal Production (EAAP), the Indian Society for the Study of Reproduction & Fertility, and the Veterinary Council of India.

Shanti Choudhary, Ph.D., served at the University of Vermont, Burlington, the USA, as a Lab Manager and Lab Safety Officer for more than a year. She has extensive research experience inmany aspects of molecular biology, including mammary stem cell physiology. She worked at the USDA in Beltsville, USA as a visiting scientist for more than two years, where her focus was on parasitic diseases in animals. She has also worked as a Research Associate at Guru Angad Dev Veterinary and Animal Sciences University. She received a National Post-doctoral Fellowship (N-PDF; SERB-DST, India) for a project on developing an in vitro model for the characterization and manipulation of buffalo mammary stem cells. She has published more than 30 research articles in peer-reviewed international journals and is a member of several scientific societies, e.g., the American Society for Biochemistry and Molecular Biology and the American Dairy Science Association. Currently, she is engaged in teaching at the Students College of Animal Biotechnology, GADVASU, Ludhiana.

Demonstrates the therapeutic potential of animal stem cells, including those of mesenchymal, mammary gland, and testicular origin

Reviews methods for the identification, characterization, and quantification of stem cells of animal origin

Summarizes future prospects and challenges of using animal stem cells in regenerative medicine

Explores the potential of genome editing in stem cell therapy for veterinary medicine

Part 1: Overview And Introduction.- 1 Overview of Stem Cells and Their Applications in Veterinary Medicine.- 2 Introduction to Mammary Gland and its Cell Types.- 3 Mammary Stem Cells: How Much Do We Know?.- 4 Methods of Identification and Characterization of Stem Cells.- 5 Potential of Stem Cell Therapy to Combat Mastitis in Dairy Animals.- PART 2: STEM CELLS AND VETERINARY RESEARCH.- 6 Fatty Liver Disease and Utility of Stem Cells in Developing the Disease Model.- 7 Mammary Epithelial Cells: A Potential Cellular Model to Understand the Impact of Heat Stress on Mammary Gland and Milk Production in Dairy Animals.- 8 Milk and Milk-Derived Stem Cells.- 9 Cryopreservation of Testicular Stem Cells and its Application in Veterinary Science.- 10 Testicular Stem Cell Niche.- 11 Proteomics of Mammary Cell Lines and Mammary Stem Cells.- PART 3: THERAPEUTIC APPLICATIONS.- 12 Advancing Quantitative Stem Cell Dosing for Veterinary Stem Cell Medicine.-13 Mesenchymal Stem Cells: a novel therapy for the treatment of Bovine Mastitis.- 14 Therapeutic Applications of Mesenchymal Stem Cells in Canine Diseases.- 15 Biomaterials and Scaffolds in Stem Cell Therapy.- 16 Prospects of Mesenchymal Stem Cell Secretome in Veterinary Regenerative Therapy.- 17 Reprogramming and Induced pluripotent stem cells in porcine.- 18 CRISPR/Cas System and Stem Cell Editing: Prospects and Possibilities in Veterinary Sciences.- PART 4: ISSUES AND PERSPECTIVES.- 19 Identification of Species-Specific Stem Cells and Challenges.- 20 Regulations of Animal Cell-Based Drugs in Veterinary Regenerative Medicine.