Features all the latest and hottest technology used in conventional and real-time RT-PCR

Provides a broad scope covered in good depth, from detection of minute qualities of microRNA to the analysis of complex cell populations

Serves as a comprehensive guide to setting up, running and analyzing an RT-PCR experiment

Part I: The RT-PCR Detective: Hunting Down the Best Method

1. Single Cell RT-PCR on Mouse Embryos: A General Approach for Developmental Biology
Wolfgang Mann and Thomas Haaf

2. Poly(A) cDNA Real-Time PCR for Indicator Gene Measurement in Cancer
Melissa Oliveira-Cunha, Ajith K. Siriwardena, and Richard J. Byers

3. Transcriptome Profiling of Host-Microbe Interactions by Differential Display RT-PCR
W. F. Leong and Vincent T. K. Chow

4. Quantitative RT-PCR Methods for Mature microRNA Expression Analysis
Stephanie D. Fiedler, Martha Z. Carletti, and Lane K. Christenson

5. Detection of Influenza A Virus Neuraminidase and PB2 Gene Segments by One Step Reverse Transcription Polymerase Chain Reaction
Alejandra Castillo Alvarez, Victoria Boyd, Richard Lai, Sandy Pineda, Hans G. Heine, and Ross Barnard

6. Detection and Identification of CD46 Splicing Isoforms by Nested RT-PCR
Anita Szalmás, József Kónya, István Sziklai, and Tamás Karosi

7. Simultaneous Detection of Bluetongue Virus RNA, Internal Control GAPDH mRNA, and External Control Synthetic RNA by Multiplex Real-Time PCR
Frank Vandenbussche, Elise Vandemeulebroucke, and Kris De Clercq

8. Detection of West Nile Viral RNA from Field-Collected Mosquitoes in Tropical Regions by Conventional and Real-Time RT-PCR
Ana Silvia González-Reiche, María de Lourdes Monzón-Pineda, Barbara W. Johnson, and María Eugenia Morales-Betoulle

9. Detection of Antisense RNA Transcripts by Strand-Specific RT-PCR
Eric C.H. Ho, Michael E. Donaldson, and Barry J. Saville

10. RT-PCR Amplification and Cloning of Large Viral Sequences
Xiaofeng Fan and Adrian M. Di Bisceglie

Part II: The RT-PCR Mathematician: Assessing Gene and RNA Expression

11. One-Step RT-LATE-PCR for mRNA and Viral RNADetection and Quantification
Cristina Hartshorn and Lawrence J. Wangh

12. Changes in Gene Expression of Caveolin-1 after Inflammatory Pain Using Quantitative Real-Time PCR
Fiza Rashid-Doubell

13. Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction
Hongxin Fan and Ryan S. Robetorye

14. The Use of Comparative Quantitative RT-PCR to Investigate the Effect of Cysteine Incubation on GPx1 Expression in Freshly Isolated Cardiomyocytes
Nicola King

15. The Renaissance of Competitive PCR as an Accurate Tool for Precise Nucleic Acid Quantification
Lorena Zentilin and Mauro Giacca

Part III: The RT-PCR Master Chef: Finding the Right Ingredients

16. Skeletal Muscle RNA Extraction in Preparation for RT-PCR
Janelle P. Mollica

17. Reverse Transcription of the Ribonucleic Acid: A First Step in RT-PCR Assay
Fadia Haddad and Kenneth M. Baldwin

18. Primer Design for RT-PCR
Kelvin Li and Anushka Brownley

19. Hot Start PCR
Natasha Paul, Jonathan Shum, and Tony Le

20. Real-time RT-PCR for Automated Detection of HIV-1 RNA During Blood Donor Screening
Jens Müller