John Joseph Milne: Protein Chromatography

Cover: 9781071633618 | Protein Chromatography | Methods and Protocols | Milne (u. a.) | Buch

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Beschreibung

This third edition expands on the previous editions with updated and new chapters on protein chromatography. Chapters detail protein stability and storage, avoiding proteolysis, protein quantitation methods, generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Protein Chromatography: Methods and Protocols, Third Edition aims to provide commonly used methods and new approaches to help both new researchers and experts expand their knowledge.

Zusammenfassung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Inhaltsverzeichnis

A Synopsis of Proteins and Their Purification.- Mixed-mode chromatography and its role in monoclonal antibody purification.- Application of Continuous Counter-current Chromatography in Protein Purification.- Chromatographic Purification of Viral Vectors for Gene Therapy Applications.- Scale-up of Protein Purification -Downstream Processing Issues.- Approaches to avoid Proteolysis During Protein Expression and Purification.- Tagging Recombinant Proteins to Enhance Solubility and Aid Purification.- Magnetic nanoparticles for protein separation and purification.- Ion-Exchange Chromatography: Basic Principles and Application.- Hydroxyapatite Chromatography.- Poly-Histidine-Tagged Protein Purification using IMAC.- Lectin Affinity Chromatography.- Single-step non-chromatographic purification of recombinant mammalian proteins using a split intein ELP tag system.- Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC/MS) Purification Systems.- Immunoprecipitation: Variations, Considerations, and Applications.- Protein Quantitation and Analysis of Purity.- Protein Extraction and Purification by Differential Solubilisation.- Protein Stability: Enhancement and Measurement.- Storage and Lyophilisation of Pure Proteins.- Strategies for the Purification of Membrane Proteins.

Details

Erscheinungsjahr:	2023
Fachbereich:	Biophysik
Genre:	Biologie , Importe
Rubrik:	Naturwissenschaften & Technik
Medium:	Buch
Reihe:	Methods in Molecular Biology
Inhalt:	x 498 S. 8 s/w Illustr. 39 farbige Illustr. 498 p. 47 illus. 39 illus. in color.
ISBN-13:	9781071633618
ISBN-10:	1071633619
Sprache:	Englisch
Ausstattung / Beilage:	HC runder Rücken kaschiert
Einband:	Gebunden
Redaktion:	Milne, John Joseph Loughran, Sinéad T
Herausgeber:	Sinéad T Loughran/John Joseph Milne
Auflage:	Third Edition 2023
Hersteller:	Springer US Springer US, New York, N.Y. Methods in Molecular Biology
Verantwortliche Person für die EU:	Springer Verlag GmbH, Tiergartenstr. 17, D-69121 Heidelberg, juergen.hartmann@springer.com
Maße:	260 x 183 x 33 mm
Von/Mit:	John Joseph Milne (u. a.)
Erscheinungsdatum:	31.08.2023
Gewicht:	1,143 kg