Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts

1. Essential Methods of Plant Sample Preparation for Light Microscopy.- 2. Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy.- 3. Chemical Fixation, Immunofluorescence, and Immunogold Labelling of Electron Microscopical Sections.- 4. Essential Methods of Plant Sample Preparation for High Resolution Scanning Electron Microscopy at Room Temperature.- 5. Fluorescence Lifetime Imaging of Plant Cell Walls.- 6. Raman Spectroscopy in Non-Woody Plants.- 7. Image Analysis: Basic Procedures for Description of Plant Structures.- 8. From Data to Illustrations: Common (Free) Tools for Proper Image Data Handling and Processing.- 9. Visualizing and Quantifying In Vivo Cortical Cytoskeleton Structure and Dynamics.- 10. Quantitative and Comparative Analysis of Global Patterns of (Microtubule) Cytoskeleton Organization with CytoskeletonAnalyzer2D.- 11. Using FM Dyes to Study Endomembranes and Their Dynamics in Plants and Cell Suspensions.- 12. Transient GeneExpression as a Tool to Monitor and Manipulate the Levels of Acidic Phospholipids in Plant Cells.- 13. The Photo-Convertible Fluorescent Protein Dendra2 Tag as a Tool to Investigate Intracellular Protein Dynamics.- 14. Cellular Force Microscopy to Measure Mechanical Forces in Plant Cells.- 15. Optical Trapping in Plant Cells.- 16. Sequential Replicas: Method for In Vivo Imaging of Plant Organ Surfaces that Undergo Deformation.- 17. Time-Lapse Imaging of Developing Shoot Meristems Using Confocal Laser Scanning Microscope.- 18. Quantifying Plant Growth and Cell Proliferation with MorphoGraphX.- 19. Kinematic Characterization of Root Growth by Means of Stripflow.- 20. Automated Acquisition and Morphological Analysis of Cell Growth Mutants in Physcomitrella patens.- 21. Live Cell Imaging of Arabidopsis Root Hairs.- 22. Morphological Analysis of Leaf Epidermis Pavement Cells with PaCeQuant.- 23. Imaging of Developing Metaxylem Vessel Elements in Cultured Hypocotyls.- 24.Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes.- 25. Plant Cell Lines in Cell Morphogenesis Research: From Phenotyping to ¿Omics.