Includes cutting-edge methods protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

An Introduction to Plant Tissue Culture: Advances and Perspectives.- Micropropagation in the XXI Century.- Cellular and Morpho-histological Foundations of in vitro Plant Regeneration.- Bacterial Endophytes in Plant Tissue Culture: Mode of Action, Detection, and Control.- Digital Photography as a Tool of Research and Documentation in Plant Tissue Culture.- Selection of Molecular Markers for the Estimation of Somaclonal Variation.- Plant Tissue Culture. A Battle Horse in the Genome Editing Using CRISPR/Cas9.- Micropropagation of Agave Species.- Protocol for the Micropropagation of Coconut from Plumule Explants.- Micropropagation of Yucca Species.- Auxin Immunolocalization in Coffea canephora Tissues.- Somatic Embryogenesis in Common Bean Phaseolus vulgaris L..- Induction of Somatic embryogenesis in Jatropha curcas.- In Vitro Proliferation of Female Buds for Induction of Somatic Embryogenesis from False Horn Plantain (AAB, Curraré).-Somatic Embryogenesis in Theobroma cacao L..- Somatic Embryogenesis of Quercus suber L. from Immature Zygotic Embryos.- Cryotherapy: a Novel Method for Virus Eradication in Economically Important Plant Species.- Cryopreservation of Pineapple Shoot Tips by the Droplet Vitrification Technique.- Cryopreservation of Pollen Grains of Pineapple and Other Bromeliads.- Application of in Casa Pollination and Embryo Rescue Techniques for Breeding of Agave Species.- Haploid and Doubled Haploid Plant Production in Carrot Using Induced Parthenogenesis and Ovule Excision in Vitro.- Using Flow Cytometry Analysis in Plant Tissue Culture Derived Plants.- Procedure for Estimating the Tolerance and Accumulation of Heavy Metals Using Plant Cell Cultures.- Proteomics as a Tool to Study Molecular Changes During Plant Morphogenesis in Vitro.- Proteomic Analysis of Non-Model Plant Tissues Using Phenol Extraction, Two-Dimensional Electrophoresis and MALDI Mass Spectrometry.- Chromatin Immunoprecipitation (ChiP) Protocol for the Analysis of Gene Regulation by Histone Modifications in Agave angustifolia Haw.- Transcription Factors: Their Role in the Regulation of Somatic Embryogenesis in Theobroma cacao L. and other Species.- MicroRNA Expression and Regulation During Maize Somatic Embryogenesis.- Elaboration of Transcriptome during the Induction of Somatic Embryogenesis.- Induction of Specialized Metabolism in in vitro Cultures of Capsicum chinense Jacq..- Analysis of Terpenoid Indole Alkaloids, Carotenoids, Phytosterols, and NMR-Based Metabolomics for Catharanthus roseus Cell Suspension Cultures..- Transformed Root Culture: from Genetic Transformation to NMR-based Metabolomics.- Genetic Transformation of Pentalinon andrieuxii Tissue Cultures.- Appendix A: The Components of the Culture Media.