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Authoritative and cutting-edge, Galectins: Methods and Protocols, Second Eidtion aims to be a useful practical guide to researches to help further their study in this field.
Authoritative and cutting-edge, Galectins: Methods and Protocols, Second Eidtion aims to be a useful practical guide to researches to help further their study in this field.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Galectins: An Ancient Family of Carbohydrate Binding Proteins with Modern Functions.- Cloning, Expression and Purification of Galectins for In Vitro Studies.- Purification of Recombinant Galectins Using Affinity Chromatography Methods.- Alkylation of Galectin-1 with Iodoacetamide and Mass Spectrometric Mapping of the Sites of Incorporation.- Rapid Detection and Purification of Galectin-3 by the Capture and Release (CaRe) Method.- Introducing 77Se NMR Spectroscopy to Analyzing Galectin-Ligand Interaction.- Evaluation of Galectin Binding by Surface Plasmon Resonance.- Revealing the Identity of Human Galectin-3 as a Glycosaminoglycan-Binding Protein.- Examining Galectin Binding Specificity Using Glycan Microarrays.- Mechanism of Mucin Recognition by Lectins - A Thermodynamic Study.- Examination of Whole Cell Galectin Binding by Solid Phase and Flow Cytometric Analysis.- Dissecting Context-Specific Galectin Binding Using Glyco-Engineered Cell Libraries.- Method for Identifying GalectinLigands on Lymphocyte Membrane Glycoproteins.- Transformation of Agrocybe Cylindracea Galectin into aGalNAc-specific Lectin.- What Happens if a Human Galectin Enters the Endoplasmic Reticulum?.- Investigation of Galectins in Frozen Tissue and Mammalian Cell Culture Using Confocal Microscopy.- Exploring the Galectin Network by Light and Fluorescence Microscopy.- Molecular Imaging for In Vivo Tracking and Detection of Galectin Binding Partners.- Visualization of Cytosolic Galectin Accumulation Around Damaged Vesicles and Organelles.- Transcytosis of Galectin-3 in Mouse Intestine.- Evaluating the Role of Galectins in Clathrin Independent Endocytosis.- Examination of Galectin-3-Recruitment into Multivesicular Bodies for Exosomal Secretion.- Manipulating Galectin Expression in Zebrafish (Danio Rerio).- Examining Galectin Gene Regulation by Reporter Assays.- Examining the Impact of Galectin-9 on Latent HIV Transcription.- Evaluation of the Role of Galectins in Parasite Immunity.- Evaluationof the Bactericidal Activity of Galectins.- Detection of Phosophatidylserine Exposure on Leukocytes Following Treatment with Human Galectins.- Detection of Reactive Oxygen Species in Human Neutrophils Under Various Conditions of Exposure to Galectin.- Analysis of Galectin-Binding Receptors on B Cells.- Methods for Assessing the Effects of Galectins on Leukocytetrafficking and Clearance.- Examination of the Contributions of Maternal/Placental-Derived Galectin-1 to Pregnancy Outcome.- Method to Study the Role of Galectins in Angiogenesis In Vivo Using the Chick Chorioallantoic Membrane Assay.- Untangling Galectin-Mediated Circuits that Control Hypoxia-Driven Angiogenesis.- Examination of the Role of Galectins and Galectin Inhibitors in Endothelial Cell Biology.- Evaluating Therapeutic Activity of Galectin-1 in Sarcolemma Repair of Skeletal Muscle.- Exploring the Role of Galectins in Cancer: In Vitro and In Vivo Approaches.
Erscheinungsjahr: | 2022 |
---|---|
Fachbereich: | Allgemeines |
Genre: | Biologie |
Rubrik: | Naturwissenschaften & Technik |
Medium: | Buch |
Seiten: | 752 |
Reihe: | Methods in Molecular Biology |
Inhalt: |
xix
730 S. 35 s/w Illustr. 86 farbige Illustr. 730 p. 121 illus. 86 illus. in color. |
ISBN-13: | 9781071620540 |
ISBN-10: | 1071620541 |
Sprache: | Englisch |
Ausstattung / Beilage: | HC runder Rücken kaschiert |
Einband: | Gebunden |
Redaktion: |
Stowell, Sean R.
Cummings, Richard D. Arthur, Connie M. |
Herausgeber: | Sean R Stowell/Connie M Arthur/Richard D Cummings |
Auflage: | 2nd ed. 2022 |
Hersteller: |
Springer US
Springer US, New York, N.Y. Methods in Molecular Biology |
Maße: | 260 x 183 x 46 mm |
Von/Mit: | Sean R. Stowell (u. a.) |
Erscheinungsdatum: | 24.03.2022 |
Gewicht: | 1,586 kg |
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Galectins: An Ancient Family of Carbohydrate Binding Proteins with Modern Functions.- Cloning, Expression and Purification of Galectins for In Vitro Studies.- Purification of Recombinant Galectins Using Affinity Chromatography Methods.- Alkylation of Galectin-1 with Iodoacetamide and Mass Spectrometric Mapping of the Sites of Incorporation.- Rapid Detection and Purification of Galectin-3 by the Capture and Release (CaRe) Method.- Introducing 77Se NMR Spectroscopy to Analyzing Galectin-Ligand Interaction.- Evaluation of Galectin Binding by Surface Plasmon Resonance.- Revealing the Identity of Human Galectin-3 as a Glycosaminoglycan-Binding Protein.- Examining Galectin Binding Specificity Using Glycan Microarrays.- Mechanism of Mucin Recognition by Lectins - A Thermodynamic Study.- Examination of Whole Cell Galectin Binding by Solid Phase and Flow Cytometric Analysis.- Dissecting Context-Specific Galectin Binding Using Glyco-Engineered Cell Libraries.- Method for Identifying GalectinLigands on Lymphocyte Membrane Glycoproteins.- Transformation of Agrocybe Cylindracea Galectin into aGalNAc-specific Lectin.- What Happens if a Human Galectin Enters the Endoplasmic Reticulum?.- Investigation of Galectins in Frozen Tissue and Mammalian Cell Culture Using Confocal Microscopy.- Exploring the Galectin Network by Light and Fluorescence Microscopy.- Molecular Imaging for In Vivo Tracking and Detection of Galectin Binding Partners.- Visualization of Cytosolic Galectin Accumulation Around Damaged Vesicles and Organelles.- Transcytosis of Galectin-3 in Mouse Intestine.- Evaluating the Role of Galectins in Clathrin Independent Endocytosis.- Examination of Galectin-3-Recruitment into Multivesicular Bodies for Exosomal Secretion.- Manipulating Galectin Expression in Zebrafish (Danio Rerio).- Examining Galectin Gene Regulation by Reporter Assays.- Examining the Impact of Galectin-9 on Latent HIV Transcription.- Evaluation of the Role of Galectins in Parasite Immunity.- Evaluationof the Bactericidal Activity of Galectins.- Detection of Phosophatidylserine Exposure on Leukocytes Following Treatment with Human Galectins.- Detection of Reactive Oxygen Species in Human Neutrophils Under Various Conditions of Exposure to Galectin.- Analysis of Galectin-Binding Receptors on B Cells.- Methods for Assessing the Effects of Galectins on Leukocytetrafficking and Clearance.- Examination of the Contributions of Maternal/Placental-Derived Galectin-1 to Pregnancy Outcome.- Method to Study the Role of Galectins in Angiogenesis In Vivo Using the Chick Chorioallantoic Membrane Assay.- Untangling Galectin-Mediated Circuits that Control Hypoxia-Driven Angiogenesis.- Examination of the Role of Galectins and Galectin Inhibitors in Endothelial Cell Biology.- Evaluating Therapeutic Activity of Galectin-1 in Sarcolemma Repair of Skeletal Muscle.- Exploring the Role of Galectins in Cancer: In Vitro and In Vivo Approaches.
Erscheinungsjahr: | 2022 |
---|---|
Fachbereich: | Allgemeines |
Genre: | Biologie |
Rubrik: | Naturwissenschaften & Technik |
Medium: | Buch |
Seiten: | 752 |
Reihe: | Methods in Molecular Biology |
Inhalt: |
xix
730 S. 35 s/w Illustr. 86 farbige Illustr. 730 p. 121 illus. 86 illus. in color. |
ISBN-13: | 9781071620540 |
ISBN-10: | 1071620541 |
Sprache: | Englisch |
Ausstattung / Beilage: | HC runder Rücken kaschiert |
Einband: | Gebunden |
Redaktion: |
Stowell, Sean R.
Cummings, Richard D. Arthur, Connie M. |
Herausgeber: | Sean R Stowell/Connie M Arthur/Richard D Cummings |
Auflage: | 2nd ed. 2022 |
Hersteller: |
Springer US
Springer US, New York, N.Y. Methods in Molecular Biology |
Maße: | 260 x 183 x 46 mm |
Von/Mit: | Sean R. Stowell (u. a.) |
Erscheinungsdatum: | 24.03.2022 |
Gewicht: | 1,586 kg |