Theam Soon Lim: Phage Display | Buch

Cover: 9781493974467 | Phage Display | Methods and Protocols | Theam Soon Lim (u. a.) | Buch

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Beschreibung

This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery. The chapters in this book are divided into four sections: 1) construction of antibody libraries; 2) selection strategies for antibodies; 3) complementary approaches for antibody selection; and 4) phage display for epitope mapping and biomarker identification. The chapters also provide a list of antibody phage display technologies and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Phage Display: Methods and Protocols will provide technical assistance to new start-ups venturing into the field of antibody phage [...] volume will also aid in stirring interest and ideas among researchers in this ever-expanding subject.

Zusammenfassung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Inhaltsverzeichnis

Construction of Human Immune and Naïve scFv Libraries.- Construction of Naïve and Immune Human Fab Phage Display Library.- Construction of Synthetic Antibody Phage Display Libraries.- Modular Construction of Large Non-Immune Human Antibody Phage Display Libraries from Variable Heavy and Light Chain Gene Cassettes.- Construction of Macaque Immune Libraries.- Construction of Bovine Immunoglobulin Libraries in the single chain Fragment variable (scFv) Format.- Construction of Rabbit Immune Antibody Libraries.- Generation of Semi-Synthetic Shark IgNAR Single Domain Antibody Libraries.- Construction of High-Quality Camel Immune Antibody Libraries.- Construction of Chicken Antibody Libraries.- Construction and Selection of Affilin Phage Display Libraries.- Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and Antibodies.- Targeting Intracellular Antigens with pMHC-Binding Antibodies: A Phage Dis

play Approach.- Parallelized Antibody Selection in Microtiter Plates.- Mass Spectrometry Immuno Assay (MSIA (TM)) Streptavidin Disposable Automation Research Tips (D.A.R.T's®) Antibody Phage Display Biopanning.- Magnetic Nanoparticle-Based Semi-Automated Panning for High-Throughput Antibody Selection.- Phage Display and Selections on Cells.- Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor Antigens.- Antibody Isolation Form a Human Synthetic Combinational and Other Libraries of Single-Chain Antibodies.- Screening Phage Display Antibody Libraries using Protein Arrays.- Antibody Selection on FFPE Tissue Slides.- Antibody Affinity and Stability Maturation by Error Prone PCR.- Upgrading Affinity Screening Experiments by Analysis of Next Generation Sequencing Data.- Next-Generation DNA Sequencing of VH/VL Repertoires: A Primer and Guide to Applications in Single-Domain Antibody Library Construction.- High-Throughput IgG Reformatting and Expressions.- Monitoring Phage Biopanning by Next-Generation Sequencing.- ORFeome Phage Display.- Epitope Mapping by Phage Display.- Metasecretome Phage Display.- Phagekines: Screening Binding Properties and Biological Activity of Functional Cytokines Displayed on Phages.

Details

Erscheinungsjahr:	2017
Fachbereich:	Therapie
Genre:	Medizin
Rubrik:	Wissenschaften
Medium:	Buch
Reihe:	Methods in Molecular Biology
Inhalt:	xiv 562 S. 17 s/w Illustr. 43 farbige Illustr. 562 p. 60 illus. 43 illus. in color.
ISBN-13:	9781493974467
ISBN-10:	1493974467
Sprache:	Englisch
Herstellernummer:	978-1-4939-7446-7
Ausstattung / Beilage:	HC runder Rücken kaschiert
Einband:	Gebunden
Redaktion:	Lim, Theam Soon Hust, Michael
Herausgeber:	Michael Hust/Theam Soon Lim
Auflage:	1st ed. 2018
Hersteller:	Springer US Springer New York Springer US, New York, N.Y. Methods in Molecular Biology
Maße:	260 x 183 x 37 mm
Von/Mit:	Theam Soon Lim (u. a.)
Erscheinungsdatum:	21.11.2017
Gewicht:	1,266 kg