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An authoritative introduction to the fast-changing world of molecular biotechnology
In continuous publication since 1994 and now in its sixth edition, Molecular Biotechnology: Principles and Applications of Recombinant DNA has been effective in introducing this complex field to students for more than 25 years. This textbook covers essentially every aspect of the field of molecular biotechnology, which is constantly changing and adapting in light of new advances.
This edition includes the latest techniques in DNA sequencing and genetic engineering of microbial, plant, and animal genomes, including human genome editing, as well as updates across many areas, such as:
- Immunological assays for disease diagnosis, more effective bacteriophage therapy, and new ways of dealing with antibiotic-resistant bacteria
- New and developing vaccines for influenza, tuberculosis, and emerging viral threats, including Zika and SARS-CoV-2
- Engineering bacteria to perform plastic degradation and green algae to produce hydrogen, altering amino acid biosynthesis, and creating designer cellulosomes
- Production of humanized monoclonal antibodies in plants, modifying hybrid plants to produce clonal hybrids, and protecting plants from viral and fungal diseases
Molecular Biotechnology features nearly 600 detailed figures and is an ideal textbook for undergraduate and graduate courses in introductory biotechnology, as well as courses dedicated to utilizing this technology, such as medical, agricultural, environmental, and industrial biotechnology applications.
An authoritative introduction to the fast-changing world of molecular biotechnology
In continuous publication since 1994 and now in its sixth edition, Molecular Biotechnology: Principles and Applications of Recombinant DNA has been effective in introducing this complex field to students for more than 25 years. This textbook covers essentially every aspect of the field of molecular biotechnology, which is constantly changing and adapting in light of new advances.
This edition includes the latest techniques in DNA sequencing and genetic engineering of microbial, plant, and animal genomes, including human genome editing, as well as updates across many areas, such as:
- Immunological assays for disease diagnosis, more effective bacteriophage therapy, and new ways of dealing with antibiotic-resistant bacteria
- New and developing vaccines for influenza, tuberculosis, and emerging viral threats, including Zika and SARS-CoV-2
- Engineering bacteria to perform plastic degradation and green algae to produce hydrogen, altering amino acid biosynthesis, and creating designer cellulosomes
- Production of humanized monoclonal antibodies in plants, modifying hybrid plants to produce clonal hybrids, and protecting plants from viral and fungal diseases
Molecular Biotechnology features nearly 600 detailed figures and is an ideal textbook for undergraduate and graduate courses in introductory biotechnology, as well as courses dedicated to utilizing this technology, such as medical, agricultural, environmental, and industrial biotechnology applications.
About the Authors
Bernard R. Glick, PhD, is a distinguished professor emeritus at the University of Waterloo, Ontario, Canada, where he was the chair of biology from 2002 to 2008. He has authored more than 400 publications that have garnered over 54,000 citations.
Cheryl L. Patten, PhD, is a professor of microbiology in the Biology Department at the University of New Brunswick, Fredericton, Canada. She teaches courses in introductory biology, microbiology, and biotechnology.
1. The Development of Molecular Biotechnology 1
2. Fundamental Technologies 13
Molecular Cloning 13
Genome Engineering Using CRISPR Technology 36
Polymerase Chain Reaction 39
Chemical Synthesis of Genes 46
DNA Sequencing Technologies 48
Sequencing Whole Genomes 56
Genomics 62
3. Production of Recombinant Proteins 91
Protein Production in Prokaryotic Hosts 91
Heterologous Protein Production in Eukaryotic Cells 119
Directed Mutagenesis 156
4. Molecular Diagnostics 179
Immunological Approaches To Detect Protein Biomarkers 180
DNA-Based Diagnostic Approaches 199
Detecting RNA Signatures of Disease 226
Biofluorescent and Bioluminescent Systems 233
5. Protein Therapeutics 243
Pharmaceuticals 244
Enzymes 253
Bacteria and Therapeutics 267
Recombinant Antibodies 280
Affibody Molecules 315
6. Nucleic Acids as Therapeutic Agents 325
Targeting Specific mRNA and DNA Sequences 327
Viral Delivery Systems 357
Nonviral Delivery Systems 365
Gene Therapy 376
7. Vaccines 387
Vaccination 387
Current and Future Vaccines 389
Subunit and Peptide Vaccines 392
Genetic Immunization: DNA Vaccines 414
Engineered Attenuated Vaccines 424
Vector Vaccines 430
Monoclonal Antibody Passive Immunity 449
8. Industrial and Environmental Uses of Recombinant Microorganisms 459
Restriction Endonucleases 459
Small Biological Molecules 461
Microbial Degradation of Xenobiotics 496
Utilization of Starch and Sugars 508
Utilization of Cellulose and Hemicellulose 518
Lipids from Cyanobacteria 534
Hydrogen Production 535
9. Large-Scale Production of Proteins and Nucleic Acids from Recombinant Microorganisms 545
Principles of Microbial Growth 547
Maximizing the Efficiency of the Fermentation Process 551
Bioreactors 561
Typical Large-Scale Fermentation Systems 565
Harvesting Microbial Cells 574
Disrupting Microbial Cells 576
Downstream Processing 57
Large-Scale Production of DNA and RNA 583
10. Genetic Engineering of Plants: Methodology 591
Plant Transformation with the Ti Plasmid of A. tumefaciens 595
Ti Plasmid-Derived Vector Systems 597
Microprojectile Bombardment 603
Chloroplast Engineering 604
Use of Reporter Genes in Transformed Plant Cells 610
Manipulation of Gene Expression in Plants 611
Production of Marker-Free Transgenic Plants 626
11. Transgenic Plants 637
Insect Resistance 637
Virus Resistance 658
Herbicide Resistance 668
Fungus and Bacterium Resistance 674
Salt and Drought Stress 682
Phytoremediation 686
Fruits and Flowers 688
Modification of Plant Nutritional Content 690
Modification of Food Plant Taste and Appearance 701
Plants as Bioreactors 706
Edible Vaccines 711
Plant Yield 716
12. Transgenic Animals 731
Transgenic Animal Methodologies 733
Transgenic Animal Models of Human Diseases 756
Animal Bioreactors for Production of Recombinant Therapeutic Proteins 767
Enhancing Production Traits of Food Animals 774
Gene Drives To Eradicate Vector-Transmitted Diseases 787
13. Molecular Biotechnology and Society 799
Development of Guidelines for Recombinant DNA Research 800
Deliberate Release of Genetically Modified Microorganisms 802
Regulation of Genetically Modified Foods 804
Societal Concerns about Genetically Modified Foods 812
Regulation and Safety of Medical Products of Biotechnology 827
Patenting Biotechnology 837
Summary 845
References 846
Review Questions 848
Amino Acids of Proteins and Their Designations 851
Index 853
| Erscheinungsjahr: | 2022 |
|---|---|
| Fachbereich: | Grundlagen |
| Genre: | Biologie, Importe |
| Rubrik: | Naturwissenschaften & Technik |
| Medium: | Buch |
| Reihe: | ASM Books |
| Inhalt: | 896 S. |
| ISBN-13: | 9781683673644 |
| ISBN-10: | 1683673646 |
| Sprache: | Englisch |
| Herstellernummer: | 1W683673640 |
| Einband: | Gebunden |
| Autor: |
Glick, Bernard R.
Patten, Cheryl L. |
| Auflage: | 6. Auflage |
| Hersteller: |
American Society for Microbiology
ASM Books |
| Verantwortliche Person für die EU: | Wiley-VCH GmbH, Boschstr. 12, D-69469 Weinheim, product-safety@wiley.com |
| Maße: | 285 x 225 x 52 mm |
| Von/Mit: | Bernard R. Glick (u. a.) |
| Erscheinungsdatum: | 03.03.2022 |
| Gewicht: | 2,095 kg |
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