David P. Clark did his graduate work on bacterial antibiotic resistance to earn his Ph.D. from Bristol University, England. He later crossed the Atlantic to work as a postdoctoral researcher at Yale University and then the University of Illinois. Dr Clark recently retired from teaching Molecular Biology and Bacterial Physiology at Southern Illinois University which he joined in 1981. His research into the Regulation of Alcohol Fermentation in E. coli was funded by the U.S. Department of Energy, from 1982 till 2007. In 1991 he received a Royal Society Guest Research Fellowship to work at Sheffield University, England while on sabbatical leave.

Unit 1: Basic Chemical and Biological Principles1. Cells and Organisms2. Basic Genetics3. DNA, RNA, and Protein4. Genes, Genomes, and DNA5. Manipulation of Nucleic Acids

Unit 2: The Genome6. The Polymerase Chain Reaction7. Cloning Genes for Analysis8. DNA Sequencing9. Genomics and Systems BiologyNew Chapter to cover metagenomics, symbiosis, epigenomics etc

Unit 3: The Central Dogma of Molecular Biology10. Cell Division and DNA Replication11. Transcription of Genes12. Processing of RNA (moved to Unit 4)13. Protein Synthesis14. Protein Structure and Function15. Proteomics: Global Analysis of Proteins

Unit 4: Regulating Gene Expression16. Regulation of Transcription in Prokaryotes17. Regulation of Transcription in Eukaryotes12. Processing of RNA (moved from Unit 3)18. Regulation at the RNA LevelNew Chapter on Genome defense (RNAi and CRISPR). Would include their use in genetic analysis and genome editing19. Analysis of Gene Expression (Transcriptome)

Unit 5: Subcellular Life Forms20. Plasmids21. Viruses22. Mobile DNA

Unit 6: Changing the DNA Blueprint23. Mutations and Repair24. Recombination25. Bacterial Genetics26. Molecular Evolution