Yilin Hu: Metalloproteins | Taschenbuch

Cover: 9781493994038 | Metalloproteins | Methods and Protocols | Yilin Hu | Taschenbuch | x

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Beschreibung

This volume provides an up-to-date, in-depth overview of the methods that have been applied to studying the complex metalloproteins at a molecular level. Chapters cover a wide range of approaches focusing on genetic, biochemical, spectroscopic, chemical methods, and theoretical calculations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Metalloproteins: Methods and Protocols aims to be useful for anyone who is interested in metalloprotein research and wants to address the unanswered mechanistic and biosynthetic questions of these fascinating enzyme systems.

Zusammenfassung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Inhaltsverzeichnis

Nitrogenases.- Bacteriochlorophyll and Coenzyme F430.- Carbon Monoxide Dehydrogenases.- Molybdenum-Containing Enzymes.- Hydrogenases.- Genomic Manipulations of the Diazotroph Azotobacter vinelandii.- Purification of Nitrogenase Proteins.- Expression, Purification and Activity Analysis of Chlorophyllide Oxidoreductase and Ni2+-Sirohydrochlorin a,c-Diamide Reductase.- Reconstitution of Molybdoenzymes with Bis-Molybdopterin Guanine Dinucleotide Cofactor.- Crystallization of Nitrogenase Proteins.- X-Ray Crystallography of Carbon Monoxide Dehydrogenases.- X-Ray Absorption Spectroscopy of Metalloproteins.- Electron Paramagnetic Resonance Spectroscopy of Metalloproteins.- Magnetic Circular Dichroism Spectroscopy of Metalloproteins.- Chemical Synthesis of an Asymmetric Mimic of the Nitrogenase Active Site.- Computational Methods for Modeling Metalloproteins.

Details

Erscheinungsjahr:	2019
Fachbereich:	Biophysik
Genre:	Biologie , Importe
Rubrik:	Naturwissenschaften & Technik
Medium:	Taschenbuch
Inhalt:	x 275 S. 22 s/w Illustr. 40 farbige Illustr. 275 p. 62 illus. 40 illus. in color.
ISBN-13:	9781493994038
ISBN-10:	1493994034
Sprache:	Englisch
Einband:	Kartoniert / Broschiert
Redaktion:	Hu, Yilin
Herausgeber:	Yilin Hu
Auflage:	Softcover reprint of the original 1st edition 2019
Hersteller:	Springer US Springer New York
Verantwortliche Person für die EU:	Springer Verlag GmbH, Tiergartenstr. 17, D-69121 Heidelberg, juergen.hartmann@springer.com
Maße:	254 x 178 x 16 mm
Von/Mit:	Yilin Hu
Erscheinungsdatum:	10.12.2019
Gewicht:	0,547 kg