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Gel Electrophoresis of Proteins
A Practical Approach
Taschenbuch von Hames
Sprache: Englisch

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Beschreibung
This new edition is almost a completely new text, with eight of the ten chapters written by new authors. It presents the most reliable methods for essential procedures such as one-dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis,
preparative gel electrophoresis, and peptide mapping, complete with the latest refinements of the procedures. In addition, it describes major new techniques developed since the previous edition. These include capillary gel electrophoresis, sequence analysis of gel-resolved proteins,
fluorophore-labelled saccharide electrophoresis, and analysis of protein-protein interactions by gel electrophoresis. Like the previous editions, this volume is easy-to-follow and thorough, a laboratory manual written by experienced researchers for researchers. The emphasis is on describing the best
methods, in step-by-step detail, with copious advice to ensure that each method works the first time. This new edition is a 'must' for anyone currently uses gel electrophoresis.
This new edition is almost a completely new text, with eight of the ten chapters written by new authors. It presents the most reliable methods for essential procedures such as one-dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis,
preparative gel electrophoresis, and peptide mapping, complete with the latest refinements of the procedures. In addition, it describes major new techniques developed since the previous edition. These include capillary gel electrophoresis, sequence analysis of gel-resolved proteins,
fluorophore-labelled saccharide electrophoresis, and analysis of protein-protein interactions by gel electrophoresis. Like the previous editions, this volume is easy-to-follow and thorough, a laboratory manual written by experienced researchers for researchers. The emphasis is on describing the best
methods, in step-by-step detail, with copious advice to ensure that each method works the first time. This new edition is a 'must' for anyone currently uses gel electrophoresis.
Inhaltsverzeichnis
  • 1: One-dimensional PAGE

  • 2: Protein detection

  • 3: Preparative gel electrophoresis

  • 4: Capillary SDS electrophoresis

  • 5: Conventional isoelectric focusing

  • 6: 2-D gel electrophoresis

  • 7: Peptide mapping

  • 8: Sequence analysis of gel-resolved proteins

  • 9: Analysis of glycoproteins

  • 10: Protein: protein interactions

  • Appendix 1 - List of suppliers

  • Appendix 2 - Bibliography of polypeptide detection methods

Details
Erscheinungsjahr: 2002
Fachbereich: Biophysik
Genre: Biologie
Rubrik: Naturwissenschaften & Technik
Medium: Taschenbuch
ISBN-13: 9780199636402
ISBN-10: 0199636400
Sprache: Englisch
Ausstattung / Beilage: Paperback
Einband: Kartoniert / Broschiert
Autor: Hames
Redaktion: Rickwood, D.
Hersteller: OUP Oxford
Maße: 234 x 156 x 20 mm
Von/Mit: Hames
Erscheinungsdatum: 05.03.2002
Gewicht: 0,57 kg
Artikel-ID: 108637689
Inhaltsverzeichnis
  • 1: One-dimensional PAGE

  • 2: Protein detection

  • 3: Preparative gel electrophoresis

  • 4: Capillary SDS electrophoresis

  • 5: Conventional isoelectric focusing

  • 6: 2-D gel electrophoresis

  • 7: Peptide mapping

  • 8: Sequence analysis of gel-resolved proteins

  • 9: Analysis of glycoproteins

  • 10: Protein: protein interactions

  • Appendix 1 - List of suppliers

  • Appendix 2 - Bibliography of polypeptide detection methods

Details
Erscheinungsjahr: 2002
Fachbereich: Biophysik
Genre: Biologie
Rubrik: Naturwissenschaften & Technik
Medium: Taschenbuch
ISBN-13: 9780199636402
ISBN-10: 0199636400
Sprache: Englisch
Ausstattung / Beilage: Paperback
Einband: Kartoniert / Broschiert
Autor: Hames
Redaktion: Rickwood, D.
Hersteller: OUP Oxford
Maße: 234 x 156 x 20 mm
Von/Mit: Hames
Erscheinungsdatum: 05.03.2002
Gewicht: 0,57 kg
Artikel-ID: 108637689
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