By John E. Simmons

Introduction
Acknowledgements
Part I. Fluid Preservation Techniques and Collections
Chapter 1. History of Fluid Preservation
Fluid Preservation in the Ancient World
History of Ethyl Alcohol
Origin of the Name Alcohol
Glass Containers
The Discovery of Preservation of Specimens in Ethyl Alcohol
Early Instructions for Preserving Specimens in Fluids
Later Instructions for Preserving Specimens in Fluids
Fluid Preserved Collections
Other Fluid Preservatives
Summary

Chapter 2. Fixation
Fixation
Formaldehyde
Origin of the Names Formaldehyde, Formol, and Formalin
Commercial Formaldehyde
Formaldehyde as a Fixative and Preservative
Fixative pH Range
Narcotization
Fixatives for Botanical Specimens
Temperature, Time, and Rates of Penetration of Fixatives
The Penetration-Fixation Paradox
Lipids and Fixation
Formaldehyde and Field Work
Paraformaldehyde
Post-Formaldehyde Fixation Washing
Unwanted Effects of Formaldehyde
Gluteraldehyde
Aldehyde Safety
Alternative and Proprietary Fixatives

Chapter 3. Preservation
Preservation without Fixation
Freezing
Transfer between Fluids
Preservative Quality
Old Fluid Preservatives
Botanical Use of Fluid Preservation
Glycerin
Isopropyl Alcohol
Fluid Preservation for DNA Extraction
Evaporation
Clearing and Staining
Anatomical and Histological Fluid Preparations
Mounting Specimens Inside Containers
Glycol, Phenol, and Phenoxetol as Preservatives
Novec Fluid
Mineral Oil
Universal Fixatives
Criteria for Evaluating Alternative Fixative and Preservative Fluids

Chapter 4. Effects of Fixatives and Preservatives on Specimens
Changes in Body Dimensions and Biomass
Changes in Color
Solvent Extraction in Fixatives and Preservatives

Chapter 5. Managing Fluid Preserved Collections
Identification of Fluid Preservatives
Checking Fluid Concentration
Re-Use of Old Alcohol
pH of Preservative Solutions
Preparing Fixatives and Preservatives
Containers and Seals
Alternatives to Glass Containers
Labeling
The Storage Environment
Topping Up and Replacing Preservatives
Why do Closures Fail?
Bacterial and Fungal Growth in Fluid Collections-Detection and Remediation
Rehydration of Fluid Preserved Specimens
Moving Collections
Exhibition of Fluid Preserved Specimens
Dealing with Old Containers and Old Specimens
Repair of Damaged Fluid Preserved Specimens
Health and Safety
Fire Prevention
Formaldehyde Safety

Chapter 6. Fluid Preserved Collections as Cultural Patrimony
Why Preserve Specimens in Fluid?
The Fluid Preserved Human
Fond Memories of Fluid Preservation
Fluid Preservation in Visual Art
Fluid Preservation in Literature
Fluid Preservation in Film
Fluid Preservation in Popular Culture
The Aesthetics of Fluid Preservation

Part II. Literature in this Field
Chapter 7. Literature Cited.
Chapter 8. Literature Reviewed but Not Cited

Part III. Reference Tables
Table 1. Fluid preservation techniques.
Table 2. Timeline of milestones in published fluid preservation techniques.
Table 3. Tissue matrix types.
Table 4. Proprietary fixatives (based on manufacturer's MSDS, advertisements, and published analyses).
Table 5. Narcotizing agents.
Table 6. Disinfectant mechanisms of some preservatives (based in part on Volk and Wheeler 1984).
Table 7. Summary of factors that affect the long-term usefulness of fluid preserved specimens (after Simmons 2002).
Table 8. Timeline of the known introduction of chemicals in fluid preservation.
Table 9. Anatomical fixation and preservation techniques.
Table 10. Clearing and staining techniques.
Table 11. Disinfectant mechanisms of some preservatives (based in part on van Dam 2003).
Table 12. Criteria for identifying alternative preservative fluids (based in part on van Dam 2003).
Table 13. Summary of fixative and preservative induced changes in invertebrates.
Table 14. Summary of fixative and preservative induced changes in invertebrates by taxonomic group.
Table 15. Summary of fixative and preservative induced changes in vertebrates by taxonomic group.
Table 16. Summary of fixative and preservative induced changes in vertebrates by taxonomic group.
Table 17. Proprietary preservatives (based on manufacturer's MSDS, advertisements, and published analyses).
Table 18. Summary of published fluid concentration and pH testing of fluid preserved collections.
Table 19. Characteristics of containers for fluid preserved specimens (based on Simmons 2002).
Table 20. Oxygen permeablility of container materials.
Table 21. Published recommendations for label substrates and inks.
Table 22. Rehydration techniques for fluid preserved specimens.
Table 23. Treatments and practices that are not recommended for fluid preserved specimens.

Appendix

Index

About the Author