Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts

CRISPR-gRNA Design.- Tracking CRISPR's Footprints.- Rapid Quantitative Evaluation of CRISPR Genome Editing by TIDE and TIDER.- Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAA.- Functional Evaluation of CRISPR Activity by the Dual-Fluorescent Surrogate System: C-Check.- CRISPR-Cas9 Delivery by Artificial Virus (RRPHC).- Production and Validation of Lentiviral Vectors for CRISPR/Cas9 Delivery.- Rapid and Simple Screening of CRISPR Guide RNAs (gRNAs) in Cultured Cells Using Adeno-Associated Viral (AAV) Vectors.- Electroporation-Based CRISPR/Cas9 Gene Editing Using Cas9 Protein and Chemically Modified sgRNAs.- Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9.- Editing the Genome of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 Ribonucleoprotein Complexes.- Conditional Gene Knockout in Human Cells with Inducible CRISPR/Cas9.- CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cells.- Rapid and Efficient Gene Deletion by CRISPR/Cas9.- Genome Editing in Mice.- CRISPR/Cas9-Mediated Gene Tagging: A Step-by-Step Protocol.- Gene Editing in Primary Cells of Cattle and Pig.- Toward In Vivo Gene Therapy Using CRISPR.- CRISPR Gene Therapy of the Eye: Targeted Knockout of Vegfa in Mouse Retina by Lentiviral Delivery.- In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection.- CRISPR-Based Lentiviral Knockout Libraries for Functional Genomic Screening and Identification of Phenotype-Related Genes.