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Authoritative and cutting-edge, Cerebral Cavernous Malformations (CCM): Methods and Protocols aims to ensure successful results in the further study of this complex disease and its pathophysiologic correlates.
Authoritative and cutting-edge, Cerebral Cavernous Malformations (CCM): Methods and Protocols aims to ensure successful results in the further study of this complex disease and its pathophysiologic correlates.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
From Genes and Mechanisms to Molecular-targeted Therapies: the Long Climb to the Cure of Cerebral Cavernous Malformation (CCM) Disease.- Incidence, Prevalence, and Clinical Presentation of Cerebral Cavernous Malformations.- Natural History, Clinical, and Surgical Management of Cavernous Malformations.- Molecular Genetics Screening of CCM patients, An Overview.- Next Generation Sequecing (NGS) Strategies for Genetic Testing and Diagnosis of Cerebral Cavernous Malformation (CCM) Disease.- Genome-wide Genotyping of Cerebral Cavernous Malformation Type 1 Individuals to Identify Genetic Modifiers of Disease Severity.- Clinical Imaging of Cerebral Cavernous Malformations: Computed Tomography and Magnetic Resonance Imaging.- Neuroradiology: Differential Diagnosis, Follow-Up, and Reporting.- Surgical Management of Brain Cavernous Malformations.- Generation of CCM Phenotype by a Human Microvascular Endothelial Model.- Isolation and Purification of Mouse Brain Endothelial Cells to Study Cerebral Cavernous Malformation Disease.- Production of KRIT1-knockout and KRIT1-knockin Mouse Embryonic Fibroblasts as Cellular Models of CCM Disease.- CRISPR/Cas9-mediated Generation of Human Endothelial Cell Knockout Models of CCM Disease.- Dissection of the Role of CCM Genes in Tubulogenesis using the Drosophila Tracheal System as a Model.- Generation and Analysis of CCM Phenotypes in C. elegans.- Generation of Transgenic Lines of Zebrafish Expressing Fluorescently-tagged CCM Proteins to Study Their Function and Subcellular Localization within the Vasculature.- Vertebrate Models to Investigate CCM Pathogenesis: The Zebrafish and Mouse Model.- Generation of Cerebral Cavernous Malformation in Neonatal Mouse Models using Inducible Cre-LoxP Strategy.- Isolation of Cerebral Endothelial Cells from CCM1/KRIT1 Null Mouse Brain.- Identification of the KRIT1 Protein by LexA-based Yeast Two-Hybrid System.- Crystallographic Studies of the Cerebral Cavernous Malformations Proteins.- Microscopy Techniques to Investigate CCM Pathogenesis.- Preparation and Analysis of Protein Extracts to Investigate CCM Pathogenesis.- Systems Wide Analysis of CCM Signaling Complex (CSC) Alterations in CCM Deficient Models using Omics Approaches.- Study of Molecular Interactions of CCM Proteins by using a GAL4-based Yeast Two-hybrid Screening.- Study of CCM Microvascular Endothelial Phenotype by an in vitro Tubule Differentiation Model.- Bidimentional in vitro Angiogenic Assays to Study CCM Pathogenesis: Endothelial Cell Proliferation and Migration.- Measurement of Endothelial Barrier Function in Mouse Models of Cerebral Cavernous Malformations using Intravital Microscopy.- Immunofluorescence of Cell-Cell and Cell-Extracellular Matrix Adhesive Defectsin in vitro Endothelial CCM Model. Juxtacrine Role of Mutant Extracellular Matrix on Wild-Type Endothelial Cells.- Detection of p62/SQSTM1Aggregates in Cellular Models of CCM Disease by Immunofluorescence.- Notch Signaling in Familial Cerebral Cavernous Malformations and Immunohistochemical Detection of cleaved Notch1 Intracellular Domain.- Measuring the Kinase Activity of GCKIII Proteins in vitro.- Spectrophotometric Method for Determining Glyoxalase 1 Activity in Cerebral Cavernous Malformation (CCM) Disease.- Fluorescence Analysis of Reactive Oxygen Species (ROS) in Cellular Models of Cerebral Cavernous Malformation Disease.- Small RNA Library Preparation for Profiling Small Non-coding RNAs in Patients Affected by Cavernous Malformations.- Affinity Purification and Preparation of Peptides for Mass Spectrometry from C. elegans.
Erscheinungsjahr: | 2020 |
---|---|
Fachbereich: | Therapie |
Genre: | Medizin |
Rubrik: | Wissenschaften |
Medium: | Buch |
Seiten: | 512 |
Reihe: | Methods in Molecular Biology |
Inhalt: |
xv
496 S. 24 s/w Illustr. 62 farbige Illustr. 496 p. 86 illus. 62 illus. in color. |
ISBN-13: | 9781071606391 |
ISBN-10: | 1071606395 |
Sprache: | Englisch |
Ausstattung / Beilage: | HC runder Rücken kaschiert |
Einband: | Gebunden |
Autor: |
Trabalzini, Lorenza
Finetti, Federica Retta, Saverio Francesco |
Redaktion: |
Trabalzini, Lorenza
Retta, Saverio Francesco Finetti, Federica |
Herausgeber: | Lorenza Trabalzini/Federica Finetti/Saverio Francesco Retta |
Auflage: | 1st ed. 2020 |
Hersteller: |
Springer US
Methods in Molecular Biology |
Maße: | 260 x 183 x 33 mm |
Von/Mit: | Lorenza Trabalzini (u. a.) |
Erscheinungsdatum: | 11.06.2020 |
Gewicht: | 1,15 kg |
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
From Genes and Mechanisms to Molecular-targeted Therapies: the Long Climb to the Cure of Cerebral Cavernous Malformation (CCM) Disease.- Incidence, Prevalence, and Clinical Presentation of Cerebral Cavernous Malformations.- Natural History, Clinical, and Surgical Management of Cavernous Malformations.- Molecular Genetics Screening of CCM patients, An Overview.- Next Generation Sequecing (NGS) Strategies for Genetic Testing and Diagnosis of Cerebral Cavernous Malformation (CCM) Disease.- Genome-wide Genotyping of Cerebral Cavernous Malformation Type 1 Individuals to Identify Genetic Modifiers of Disease Severity.- Clinical Imaging of Cerebral Cavernous Malformations: Computed Tomography and Magnetic Resonance Imaging.- Neuroradiology: Differential Diagnosis, Follow-Up, and Reporting.- Surgical Management of Brain Cavernous Malformations.- Generation of CCM Phenotype by a Human Microvascular Endothelial Model.- Isolation and Purification of Mouse Brain Endothelial Cells to Study Cerebral Cavernous Malformation Disease.- Production of KRIT1-knockout and KRIT1-knockin Mouse Embryonic Fibroblasts as Cellular Models of CCM Disease.- CRISPR/Cas9-mediated Generation of Human Endothelial Cell Knockout Models of CCM Disease.- Dissection of the Role of CCM Genes in Tubulogenesis using the Drosophila Tracheal System as a Model.- Generation and Analysis of CCM Phenotypes in C. elegans.- Generation of Transgenic Lines of Zebrafish Expressing Fluorescently-tagged CCM Proteins to Study Their Function and Subcellular Localization within the Vasculature.- Vertebrate Models to Investigate CCM Pathogenesis: The Zebrafish and Mouse Model.- Generation of Cerebral Cavernous Malformation in Neonatal Mouse Models using Inducible Cre-LoxP Strategy.- Isolation of Cerebral Endothelial Cells from CCM1/KRIT1 Null Mouse Brain.- Identification of the KRIT1 Protein by LexA-based Yeast Two-Hybrid System.- Crystallographic Studies of the Cerebral Cavernous Malformations Proteins.- Microscopy Techniques to Investigate CCM Pathogenesis.- Preparation and Analysis of Protein Extracts to Investigate CCM Pathogenesis.- Systems Wide Analysis of CCM Signaling Complex (CSC) Alterations in CCM Deficient Models using Omics Approaches.- Study of Molecular Interactions of CCM Proteins by using a GAL4-based Yeast Two-hybrid Screening.- Study of CCM Microvascular Endothelial Phenotype by an in vitro Tubule Differentiation Model.- Bidimentional in vitro Angiogenic Assays to Study CCM Pathogenesis: Endothelial Cell Proliferation and Migration.- Measurement of Endothelial Barrier Function in Mouse Models of Cerebral Cavernous Malformations using Intravital Microscopy.- Immunofluorescence of Cell-Cell and Cell-Extracellular Matrix Adhesive Defectsin in vitro Endothelial CCM Model. Juxtacrine Role of Mutant Extracellular Matrix on Wild-Type Endothelial Cells.- Detection of p62/SQSTM1Aggregates in Cellular Models of CCM Disease by Immunofluorescence.- Notch Signaling in Familial Cerebral Cavernous Malformations and Immunohistochemical Detection of cleaved Notch1 Intracellular Domain.- Measuring the Kinase Activity of GCKIII Proteins in vitro.- Spectrophotometric Method for Determining Glyoxalase 1 Activity in Cerebral Cavernous Malformation (CCM) Disease.- Fluorescence Analysis of Reactive Oxygen Species (ROS) in Cellular Models of Cerebral Cavernous Malformation Disease.- Small RNA Library Preparation for Profiling Small Non-coding RNAs in Patients Affected by Cavernous Malformations.- Affinity Purification and Preparation of Peptides for Mass Spectrometry from C. elegans.
Erscheinungsjahr: | 2020 |
---|---|
Fachbereich: | Therapie |
Genre: | Medizin |
Rubrik: | Wissenschaften |
Medium: | Buch |
Seiten: | 512 |
Reihe: | Methods in Molecular Biology |
Inhalt: |
xv
496 S. 24 s/w Illustr. 62 farbige Illustr. 496 p. 86 illus. 62 illus. in color. |
ISBN-13: | 9781071606391 |
ISBN-10: | 1071606395 |
Sprache: | Englisch |
Ausstattung / Beilage: | HC runder Rücken kaschiert |
Einband: | Gebunden |
Autor: |
Trabalzini, Lorenza
Finetti, Federica Retta, Saverio Francesco |
Redaktion: |
Trabalzini, Lorenza
Retta, Saverio Francesco Finetti, Federica |
Herausgeber: | Lorenza Trabalzini/Federica Finetti/Saverio Francesco Retta |
Auflage: | 1st ed. 2020 |
Hersteller: |
Springer US
Methods in Molecular Biology |
Maße: | 260 x 183 x 33 mm |
Von/Mit: | Lorenza Trabalzini (u. a.) |
Erscheinungsdatum: | 11.06.2020 |
Gewicht: | 1,15 kg |