John T. Tansey received his B.S. in Biochemistry and Molecular Biology from the University of Massachusetts, Amherst, and his PhD in Biochemistry from Wake Forest University. John has been teaching for the past seventeen years in the Chemistry department and Biochemistry and Molecular Biology program at Otterbein University, a primarily undergraduate institution outside Columbus, Ohio. He has also taught at the community college and graduate levels. John was awarded both the Best New Teacher and Master Teacher awards from his university. He is an education fellow of the American Society for Biochemistry and Molecular Biology (ASBMB) and is a member of the editorial board of Biochemistry and Molecular Biology Education (BAMBEd).

1 The Chemical Foundations of Biochemistry 1

2 Nucleic Acids 27

3 Proteins I: An Introduction to Protein Structure, Function and Purification 69

4 Proteins II: Enzymes, Allosterism and Receptor-Ligand Interactions 133

5 Membranes and an Introduction to Signal Transduction 193

6 Carbohydrates I: Mono- and Disaccharides, Glycolysis, Gluconeogenesis, and the Fates of Pyruvate 217

7 The Common Catabolic Pathway 269

8 Carbohydrates II 315

9 Lipids I: Fatty Acids, Steroids, and Eicosanoids; Beta-Oxidation and Fatty Acid Biosynthesis 363

10 Lipids II: Metabolism and Transport of Complex Lipids 415

11 Amino Acid and Amine Metabolism 461

12 Regulation and Integration of Metabolism 505

13 Nucleotide and Deoxynucleotide Metabolism 541

14 DNA Replication, Damage, and Repair 571

15 RNA Synthesis and Processing 611

16 Protein Biosynthesis 643

17 Control and Regulation of Gene Expression 673

18 Determination of Macromolecular Structure 715

19 Protein Production, Folding, and Engineering 745

20 Bioinformatics and Omics 775

21 Signal Transduction 801

22 Protein Trafficking 833

23 Photosynthesis and Nitrogen Fixation 853

TECHNIQUES T-1

SOLUTIONS A-1

GLOSSARY G-1

INDEX I-1