Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Archaea: a goldmine for molecular biologists and evolutionists.- Progress and challenges in archaeal genetic manipulation.- Genetic manipulation of Haloferax species.- CRISPR interference as a tool to Repress Gene Expression in Haloferax volcanii.- Transformation Techniques for the Anaerobic Hyperthermophile Thermococcus kodakarensis.- Genetic Methods and Construction of Chromosomal Mutations in Methanogenic Archaea.- An interdomain conjugation protocol for plasmid-DNA transfer into Methanothermobacter thermautotrophicus ¿H.- Methods for Markerless Gene Deletion and Plasmid-Based Expression in Sulfolobus acidocaldarius.- A Rapid Targeted Gene Inactivation Approach in Sulfolobus islandicus.- Transposon Insertion Mutagenesis in hyperthermophilic crenarchaeon Sulfolobus islandicus.- Reprogramming CRISPR-mediated RNA interference for silencing of essential genes in Sulfolobales.- Progress and challenges in archaeal molecular biology.- ChIP-seq Occupancy Mapping of the Archaeal Transcription Machinery.- Conversion of ribosome-protected mRNA to DNA and deep sequencing for ribosome profiling in Haloferax volcanii.- Small RNA-sequencing library preparation for the halophilic archaeon Haloferax volcanii.- Quantitative Mass Spectrometry by SILAC in Haloferax volcanii.- Proteome turnover analysis in Haloferax volcanii by a heavy isotope multilabelling approach.- Proteomic sample preparation and data analysis in line with the Archaeal Proteome.- In vivo protein crosslinking and co-immunoprecipitation in Haloferax volcanii.- Proteolytic activity assays in haloarchaea.- Carotenoids from Haloarchaea: Extraction, fractionation and characterization.- Metal nanoparticles biosynthesis using the halophilic archaeon Haloferax volcanii.- Simplified enzymatic synthesis of 2-keto-3-deoxy-D-gluconate from D-gluconate using the gluconate dehydratase from Thermoproteus tenax.- Progress and Challenges in Archaeal Cellular Biology Analysis.- Methods to Analyze Motility in Eury- And Crenarchaea.- Immersed Liquid Biofilm and Honeycomb Pattern Formations in Haloferax volcanii.- Cost-effective and Versatile Analysis of Archaeal Surface Adhesion Under Shaking and Standing Conditions.- Cell Adhesion and Biofilm Formation Analysis.- BrdU incorporation and labelling of nascent DNA to Investigate Archaeal Replication using super-resolution imaging.- Isolation, Purification, and Characterization of Membrane Vesicles from Haloarchaea.- Archaeal Viruses: Production of Virus Particles and Vesicle-Like Viruses and Purification Using Asymmetrical Flow Field-Flow Fractionation.- Progress and Challenges in Studying the Ecophysiology of Archaea.- Reconstruction of Archaeal Genomes Assembly from Short-Read Metagenomes.- Outreach: Accessible and Insightful Scientific Learning Experiences Using the Microorganism Haloferax volcanii.