Animal Cell Culture: Principles and Practice

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Beschreibung

This introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more.

Über den Autor

Dr. Shalini Mani is Associate Professor in the Department of Biotechnology at Jaypee Institute of Information Technology, Noida, India. Dr. Manisha Singh is Assistant Professor and Lecturer in the Department of Biotechnology at Jaypee Institute of Information Technology, Noida, India. Dr. Anil Kumar is Director of Education and Professor at RLB Central Agricultural University, Jhansi, India.

Zusammenfassung

Includes best practices and lab safety, ethical considerations, and troubleshooting for common issues

Perfect for junior researchers, research students, and established scientists seeking a go-to quick guide

One-stop reference or standard animal cell culture techniques

Inhaltsverzeichnis

1. Overview to animal cell culture.- 2. Cell culture laboratory.- 3. Good lab practices and bio-safety measures.- 4. Managing sterility in the lab.- 5. Media and buffer preparation for cell culture.- 6. Properties of cultured cells and selection of culture media.- 7. Selection and maintenance of cultured cells.- 8. Inoculation and passaging of adherent cells.- 9. Counting of cells.- 10. Cryopreservation of cell lines.- 11. Resuscitation of frozen cell line.- 12. Isolation and culturing of cells from different tissues.- 13. Differentiation, transformation and immortalization of cells.- 14. Co-culture techniques.- 15. 3d cell culture techniques.- 16. Stem cell culture techniques.- 17. Identification and removal of biological contamination in the media and cell suspensions.- 18. Analysis of cell growth kinetics in suspension and adherent types cell line.- 19. Applications of animal cell culture based assays.- 20. Ethical issues in animal cell culture.- 21. Common troubleshooting methods in cell culture techniques.

Details

Erscheinungsjahr:	2023
Fachbereich:	Allgemeines
Genre:	Biologie
Rubrik:	Naturwissenschaften & Technik
Medium:	Buch
Seiten:	344
Reihe:	Techniques in Life Science and Biomedicine for the Non-Expert
Inhalt:	xvi 327 S. 6 s/w Illustr. 85 farbige Illustr. 327 p. 91 illus. 85 illus. in color.
ISBN-13:	9783031194849
ISBN-10:	3031194845
Sprache:	Englisch
Ausstattung / Beilage:	HC runder Rücken kaschiert
Einband:	Gebunden
Autor:	Mani, Shalini Kumar, Anil Singh, Manisha
Auflage:	1st ed. 2023
Hersteller:	Springer International Publishing Springer International Publishing AG Techniques in Life Science and Biomedicine for the Non-Expert
Maße:	241 x 160 x 25 mm
Von/Mit:	Shalini Mani (u. a.)
Erscheinungsdatum:	02.02.2023
Gewicht:	0,682 kg

preigu-id: 124663406