Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: [...]

Challenges in Veterinary Vaccine Development and Immunization.- Development of
Mycoplasmahyopneumoniae
Recombinant Vaccines.- Computational Prediction of Immunodominant Epitopes on Outer Membrane Protein (Omp) H of
Pasteurella Multocida
towards Designing of a Peptide Vaccine.- DNA Vaccines against Maedi-Visna Virus.- Detection of Avian Antigen-Specific T cells Induced by Viral Vaccines.- Generation of Newcastle Disease Virus (NDV) Recombinants Expressing the Infectious Laryngotracheitis Virus (ILTV) Glycoprotein gB or gD as Dual Vaccines.-

A Reverse Genetics Approach for the Design of Methyltransferase-Defective Live Attenuated Avian Metapneumovirus Vaccines.- Development of Fasciola Vaccine in an Animal Model.- Development of Experimental Vaccines against Liver Flukes.- Towards a Preventive Strategy for Toxoplasmosis: Current Trends, Challenges, and Future Perspectives for Vaccine Development.- DNA Vaccination in Chickens.- Selection of Vaccine Candidatesfor Fish Pasteurellosis Using a Reverse Vaccinology and an In Vitro Screening Approach.- Development of Vaccines against Nocardia in Fishes.- Design of an Immersion Vaccine against Aeromonad Septicaemia in Perch (
Perca fluviatilis
L.)..- Prokaryotic Production of Virus-Like Particle Vaccine of Betanodavirus.- Design and Construction of Shrimp Antiviral DNA Vaccines Expressing Long and Short Hairpins for Protection by RNA Interference.- Developing Anti-Tick Vaccines.- Host Immunization with Recombinant Proteins to Screen Antigens for Tick Control.- Vaccinomics Approach to Tick Vaccine Development.- Development of CpG ODN Based Vaccine Adjuvant Formulations.- Assembly and Assessment of DNA Scaffolded Vaccines.- Alphavirus-based Vaccines.- Vaccine Design: Replication-Defective Adenovirus Vectors.- Generation of Lymphocytic Choriomeningitis Virus based Vaccine Vectors.- Production of Japanese Encephalitis Virus-like Particles Using Insect Cell Expression Systems.- ;Subunit Protein Vaccine Delivery System for Tuberculosis Based on Hepatitis B Virus Core VLP (HBc-VLP) Particles.- Formulation Studies during Preclinical Development of Influenza Hemagglutinin and Virus-like Particle Vaccine Candidates.- Strategies for Vaccine Design using Phage Display-Derived Peptides.- Production of Well-Characterized Virus-Like Particles in an
Escherichia coli
based Expression Platform for Preclinical Vaccine Assessments.- Laboratory Scale Production of Recombinant Haa86 Tick Protein in
Pichia pastoris
and in
Escherichia coli
System.- E. Coli.- Immunoproteomic Approach for Screening Vaccine Candidates from Bacterial Outer Membrane Proteins.- Construction and Immunogenicity Testing of Whole Recombinant Yeast-Based T cell Vaccines.- Oral Rabies Vaccine Design For Expression in Plants.- Purification of Virus-like Particles (VLPs) from Plants.- Transient Expression of Viral Proteins in Plants using
Agrobacterium tumefaciens.-
A Plant-Based Transient Expression System for the Rapid Production of Malaria Vaccine Candidates.- Recombinant Botulinum Toxoids: A Practical Guide for Production.- Practical Approaches to Forced Degradation Studies of Vaccines.- Preparation of Multifunctional Liposomes as a Stable Vaccine Delivery-Adjuvant System by Procedure of Emulsification-Lyophilization.- Preparation of the Multifunctional Liposome-Containing Microneedle Arrays as an Oral Cavity Mucosal Vaccine Adjuvant-Delivery System.- Preparation and Characterization of PLGA Encapsulated Protective Antigen Domain 4 Nanoformulation.- Attenuated
Salmonella
sp. as a DNA delivery system for
T. cruzi
antigens.- Poly-e-Caprolactone/Chitosan and Chitosan Particles: Two Recombinant Antigen Delivery Systems for Intranasal Vaccination.- Micro-Fractional Epidermal Powder Delivery for Skin Vaccination.- A Bioinformatics Method for the Design of Live Attenuated Virus Vaccine Utilizing Host microRNA Response Elements.- The Web-based DNA Vaccine Database DNAVaxDB and its Usage for Rational DNA Vaccine Design.- MetaMHCpan, a Meta Approach for Pan-Specific MHC Peptide Binding Prediction.- A Cohesive and Integrated Platform for Immunogenicity Prediction.- The Regulatory Evaluation of Vaccines for Human Use.- Vaccines and IP Rights: A Multifaceted Relationship.- From the Bench to the Pharmacy: Protecting Innovation during Vaccine Development and Commercialization.- Intellectual Property in Vaccine Innovation: Impact of Recent Patent Developments