Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: [...]

Clinical Impact of Vaccine Development.- Future Challenges for Vaccinologists.- Principles of Vaccination.- Reverse Vaccinology: The Pathway from Genomes and Epitope Predictions to Tailored Recombinant Vaccines.- Systems Vaccinology: Applications, Trends, and Perspectives.- Proteomic Monitoring of B Cell Immunity.- Development of Rabies Virus-Like Particles for Vaccine Applications: Production, Characterization and Protection Studies.- Analytic Vaccinology: Antibody-Driven Design of a Human Cytomegalovirus Subunit Vaccine.- Generation of a Single-Cycle Replicable Rift Valley Fever Vaccine.- Application of Droplet Digital PCR to Validate Rift Valley Fever Vaccines.- Methods to Evaluate Novel Hepatitis C Virus Vaccines.- Designing Efficacious Vesicular Stomatitis Virus-Vectored Vaccines against Ebola Virus.- Assessment of Functional Norovirus Antibody Responses by Blocking Assay in Mice.- Development of a SARS Coronavirus Vaccine from Recombinant Spike Protein plus Delta Inulin Adjuvant.- Generation and Characterization of a Chimeric Tick-Borne Encephalitis Virus Attenuated Strain ChinTBEV.- Single-Vector, Single-injection Recombinant Vesicular Stomatitis Virus Vaccines against High Containment Viruses.- Reverse Genetics Approaches to Control Arenavirus.- DNA Vaccines: A Strategy for Developing Novel Multivalent TB Vaccines.- Overcoming Enterotoxigenic
Escherichia coli
Pathogen Diversity: Translational Molecular Approaches to Inform Vaccine Design.- Design and Purification of Subunit Vaccines for Prevention of
Clostridium difficile
Infection.- The Design of a
Clostridium difficile
Carbohydrate-based Vaccine.- Murine Models of Bacteremia and Surgical Wound Infection for the Evaluation of
Staphylococcus aureus
Vaccine Candidates.- Using MHC Molecules to define a
Chlamydia
T Cell Vaccine .- An Approach to Identify and Characterize a Subunit Candidate Shigella Vaccine Antigen.- Approach to the Discovery, Development and Evaluation of a Novel
Neisseria meningitidis
Serogroup B Vaccine.- Anti-Lyme Subunit Vaccines: Design and Development of Peptide-based Vaccine Candidates.- Assessment of Live Plague Vaccine Candidates.- Highly Effective Soluble and Bacteriophage T4 Nanoparticle Plague Vaccines against
Yersinia pestis
.- Development of Structure-based Vaccines for Ehrlichiosis.- Dendritic Cell-based Vaccine against Fungal Infection.- Flow Cytometric Analysis of Protective T-cell Response against Pulmonary
Coccidioides
Infection.- High-Density Peptide Arrays for Malaria Vaccine Development.- Development and Assessment of Transgenic Rodent Parasites for the Pre-clinical Evaluation of Malaria Vaccines.- DNA Integration in
Leishmania
Genome: an Application for Vaccine Development and Drug Screening.- Methods to Evaluate the Preclinical Safety and Immunogenicity of Genetically Modified Live Attenuated Leishmania Parasite Vaccines.- The Use of Microwave Assisted Solid Phase Peptide Synthesisand Click Chemistry for the Synthesis of Vaccine Candidates against Hookworm Infection.- Methods and Protocols for Developing Prion Vaccines.- Ricin-Holotoxin Based Vaccines: Induction of Potent Ricin Neutralizing Antibodies.- Synthesis of Hapten-protein Conjugate Vaccines with Reproducible Hapten Densities.- Production of Rice Seed-based Allergy Vaccines.- Allergy Vaccines Using a Mycobacterium-Secreted Antigen, Ag85B, and an IL-4 Antagonist.- Development of House Dust Mite Vaccine.- Cancer Vaccines: A Brief Overview.- Dendritic Cell Vaccines.- Peptide-based Cancer Vaccine Strategies and Clinical Results.- Preconditioning Vaccine Sites for mRNA--Transfected Dendritic Cell Therapy and Antitumor Efficacy.- Development of Antibody based Vaccines Targeting the Tumor Vasculature.