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The Blood-Brain Barrier
Methods and Protocols
Taschenbuch von Nicole Stone
Sprache: Englisch

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Beschreibung
This detailed volume features techniques to explore the complex interface that separates the systemic circulation from the central nervous system, known as the blood-brain barrier (BBB). Beginning with an introduction to its physiology, the book continues with sections on using pluripotent stem cells in models of the BBB, co-culture, permeability and transwell models, microfluidic and chip models, as well as models to study specific BBB pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, The Blood-Brain Barrier: Methods and Protocols collects a wide range of methodologies which will aid all researchers in the fascinating world of the blood-brain barrier.
This detailed volume features techniques to explore the complex interface that separates the systemic circulation from the central nervous system, known as the blood-brain barrier (BBB). Beginning with an introduction to its physiology, the book continues with sections on using pluripotent stem cells in models of the BBB, co-culture, permeability and transwell models, microfluidic and chip models, as well as models to study specific BBB pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, The Blood-Brain Barrier: Methods and Protocols collects a wide range of methodologies which will aid all researchers in the fascinating world of the blood-brain barrier.
Zusammenfassung

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts

Inhaltsverzeichnis

Cells of the Blood-Brain Barrier: An Overview of the Neurovascular Unit in Health and Disease.- In Vitro Models of the Blood-Brain Barrier.- In Vitro Models of the Human Blood-Brain Barrier Based on Human Induced Pluripotent Stem Cells: Opportunities and Challenges.- Induced Pluripotent Stem Cell (iPSC)-Derived Endothelial Cells to Study Bacterial-Brain Endothelial Cell Interactions.- An hiPSC-Derived In Vitro Model of the Blood-Brain Barrier.- A Three-Dimensional Brain-on-Chip Using Human iPSC-Derived GABAergic Neurons and Astrocytes.- An Improved In Vitro Porcine Blood-Brain Barrier Model for Permeability Screening and Functional Studies.- Establishment of an In VitroModel of Human Blood-Brain Barrier to Study the Impact of Ischaemic Injury.- A Novel Dynamic Human In Vitro Model for Studying the Blood-Brain Barrier.- A Dynamic, In Vitro BBB Model to Study the Effects of Varying Levels of Shear Stress.- Novel, Emerging Chip Models of the BBB and Future Directions.- Integrating Primary Astrocytes in a Microfluidic Model of the Blood-Brain Barrier.- Fabrication of Microtube-Embedded Chip to Mimic Blood-Brain Barrier Capillary Vessels.- BBB-on-a-Chip: Modeling Functional Human BBB by Mimicking 3D Brain Angiogenesis Using Microfluidic Chip.- Cell Interplay Model to Assess the Impact of Glioma Cells on Blood-Brain Barrier Permeability.- An In VitroHuman Blood-Brain Barrier Model to Study Breast Cancer Brain Metastasis.- An In Vivo Mouse Model to Study Blood-Brain Barrier Destabilization in the Chronic Phase of Stroke.- Immunohistochemical Analysis of Tight Junction Proteins.- An In Vitro BBB Model to Study Firm Shear-Stress Resistant Leukocyte Adhesion to Human Brain Endothelial Cells.- An In Vitro Model of the Blood-Brain Barrier (BBB) to Study Alzheimer's Disease: The Role of ß-Amyloid and Its Influence on PBMC Infiltration.

Details
Erscheinungsjahr: 2023
Fachbereich: Therapie
Genre: Medizin
Rubrik: Wissenschaften
Medium: Taschenbuch
Seiten: 372
Reihe: Methods in Molecular Biology
Inhalt: xi
357 S.
10 s/w Illustr.
73 farbige Illustr.
357 p. 83 illus.
73 illus. in color.
ISBN-13: 9781071622919
ISBN-10: 1071622919
Sprache: Englisch
Ausstattung / Beilage: Paperback
Einband: Kartoniert / Broschiert
Redaktion: Stone, Nicole
Herausgeber: Nicole Stone
Auflage: 1st ed. 2022
Hersteller: Springer US
Springer US, New York, N.Y.
Methods in Molecular Biology
Maße: 254 x 178 x 21 mm
Von/Mit: Nicole Stone
Erscheinungsdatum: 25.06.2023
Gewicht: 0,699 kg
preigu-id: 126987049
Zusammenfassung

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts

Inhaltsverzeichnis

Cells of the Blood-Brain Barrier: An Overview of the Neurovascular Unit in Health and Disease.- In Vitro Models of the Blood-Brain Barrier.- In Vitro Models of the Human Blood-Brain Barrier Based on Human Induced Pluripotent Stem Cells: Opportunities and Challenges.- Induced Pluripotent Stem Cell (iPSC)-Derived Endothelial Cells to Study Bacterial-Brain Endothelial Cell Interactions.- An hiPSC-Derived In Vitro Model of the Blood-Brain Barrier.- A Three-Dimensional Brain-on-Chip Using Human iPSC-Derived GABAergic Neurons and Astrocytes.- An Improved In Vitro Porcine Blood-Brain Barrier Model for Permeability Screening and Functional Studies.- Establishment of an In VitroModel of Human Blood-Brain Barrier to Study the Impact of Ischaemic Injury.- A Novel Dynamic Human In Vitro Model for Studying the Blood-Brain Barrier.- A Dynamic, In Vitro BBB Model to Study the Effects of Varying Levels of Shear Stress.- Novel, Emerging Chip Models of the BBB and Future Directions.- Integrating Primary Astrocytes in a Microfluidic Model of the Blood-Brain Barrier.- Fabrication of Microtube-Embedded Chip to Mimic Blood-Brain Barrier Capillary Vessels.- BBB-on-a-Chip: Modeling Functional Human BBB by Mimicking 3D Brain Angiogenesis Using Microfluidic Chip.- Cell Interplay Model to Assess the Impact of Glioma Cells on Blood-Brain Barrier Permeability.- An In VitroHuman Blood-Brain Barrier Model to Study Breast Cancer Brain Metastasis.- An In Vivo Mouse Model to Study Blood-Brain Barrier Destabilization in the Chronic Phase of Stroke.- Immunohistochemical Analysis of Tight Junction Proteins.- An In Vitro BBB Model to Study Firm Shear-Stress Resistant Leukocyte Adhesion to Human Brain Endothelial Cells.- An In Vitro Model of the Blood-Brain Barrier (BBB) to Study Alzheimer's Disease: The Role of ß-Amyloid and Its Influence on PBMC Infiltration.

Details
Erscheinungsjahr: 2023
Fachbereich: Therapie
Genre: Medizin
Rubrik: Wissenschaften
Medium: Taschenbuch
Seiten: 372
Reihe: Methods in Molecular Biology
Inhalt: xi
357 S.
10 s/w Illustr.
73 farbige Illustr.
357 p. 83 illus.
73 illus. in color.
ISBN-13: 9781071622919
ISBN-10: 1071622919
Sprache: Englisch
Ausstattung / Beilage: Paperback
Einband: Kartoniert / Broschiert
Redaktion: Stone, Nicole
Herausgeber: Nicole Stone
Auflage: 1st ed. 2022
Hersteller: Springer US
Springer US, New York, N.Y.
Methods in Molecular Biology
Maße: 254 x 178 x 21 mm
Von/Mit: Nicole Stone
Erscheinungsdatum: 25.06.2023
Gewicht: 0,699 kg
preigu-id: 126987049
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