Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts

Immunochemistry Analysis Using Chromogenic Substrates on Tissue Sections.- Indirect Immunofluorescence of Tissue Sections.- Flow Cytometry for Beginners: Hints and Tips for Approaching the Very First Single-Cell Technique.- High-Dimensional Immunophenotyping with 37-Color Panel Using Full-Spectrum Cytometry.- Detection of Cytokine Secreting Cells by Enzyme-Linked Immunospot (ELISpot).- Detection and Enumeration of Cytokine Secreting Cells by FluoroSpot.- Single Cell Protein Profiling by Microdroplet Barcoding and Next Generation Sequencing.- Single Cell Proteomics Using Multiplexed Isobaric Labeling for Mass Spectrometric Analysis.- Customizing Maxpar Direct Immune Profiling Assay with Additional Surface Marker and Intracellular Cytokine Staining Workflows for Expanded Mass Cytometry Panels.- High-Dimensional Tissue Profiling by Multiplexed Ion Beam Imaging.- Ultra-Sensitive Quantification of Protein and mRNA in Single Mammalian Cells with Digital PLA.- High-Throughput Multimodal Single-Cell Targeted DNA and Surface Protein Analysis Using the Mission Bio Tapestri Platform.- Simultaneous Analysis of Single-Cell Transcriptomes and Cell Surface Protein Expression of Human Hematopoietic Stem Cells and Progenitors Using the 10x Genomics Platform.- Generation of Centered Log-Ratio Normalized Antibody-Derived Tag Counts from Large Single-Cell Sequencing Datasets.- Simultaneous Quantification of Single-Cell Proteomes and Transcriptomes in Integrated Fluidic Circuits.- Combined Measurement of RNA and Protein Expression on a Single-Cell Level.- Simultaneous DNA, RNA, and Protein Analysis from Single Cells Using a High-Throughput Microfluidic Workflow for Resolution of Genotype-to-Phenotype Modalities.