Single Cell 'Omics of Neuronal Cells

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Beschreibung

This volume discusses the latest techniques used in the diverse fields of single cell ¿omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical amplification to mass spectrometry and nanotechnology for the analysis of the chemical constituents of cells. In the Neuromethods series style, chapters include both in-depth overviews, as well as detailed protocols that provide the key advice from specialists you need to get successful results in your laboratory.

Cutting-edge and comprehensive, Single Cell ¿Omics of Neuronal Cells is a valuable resource for experienced and novice researchers interested in learning more about this field and its future developments.

Zusammenfassung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Inhaltsverzeichnis

Singe Cell aRNA-Seq for Transcriptomics and Cellular Phenotyping.- Single Cell Genomic and Epigenomic DNA Analyses.- Single-Cell Proteome Profiling of Neuronal Cells.- Joint Analysis of Transcriptome and Proteome Measurements in Single Cells with TotalVI.- Single-Cell Mass Spectrometry of Metabolites and Proteins for Systems and Functional Biology.- High-Throughout Image-Guided Microprobe Mass Spectrometric Analysis of Single Cells.- Single-Cell Resolution Immunoblotting.- Quantifying Enzyme Activity and Gene Expression within Single-Cells using a Multiplexed Capillary Electrophoresis Platform.- Quantitative Chemical Imaging at the Cell Level-SIMS, Fluorescence, and Correlative Techniques.- Electrophysiology and Single Cells.- Genetically Encoded Biosensors Reveal Spatiotemporal Dynamics and Cellular Heterogeneity of Neuronal Cells.

Details

Erscheinungsjahr:	2023
Fachbereich:	Therapie
Genre:	Importe , Medizin
Rubrik:	Wissenschaften
Medium:	Taschenbuch
Inhalt:	xiv 294 S. 2 s/w Illustr. 56 farbige Illustr. 294 p. 58 illus. 56 illus. in color.
ISBN-13:	9781071625279
ISBN-10:	1071625276
Sprache:	Englisch
Einband:	Kartoniert / Broschiert
Redaktion:	Sweedler, Jonathan V. Fraser, Scott E. Eberwine, James
Herausgeber:	Jonathan V Sweedler/James Eberwine/Scott E Fraser
Auflage:	1st edition 2022
Hersteller:	Springer US
Verantwortliche Person für die EU:	Springer Verlag GmbH, Tiergartenstr. 17, D-69121 Heidelberg, juergen.hartmann@springer.com
Maße:	254 x 178 x 17 mm
Von/Mit:	Jonathan V. Sweedler (u. a.)
Erscheinungsdatum:	12.08.2023
Gewicht:	0,583 kg