Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible result

Contains key notes and implementation advice from the experts

Mutational Analysis of Protein Folding Transition States: Phi-values.- Engineered Metal-Binding Sites to Probe Protein Folding Transition States: Psi Analysis.- Site-Specific Interrogation of Protein Structure and Stability.- Purification and Handling of the Chaperonin GroEL.- ­Folding free energy surfaces from Differential Scanning Calorimetry.- Fast Folding Kinetics using Nanosecond Laser-Induced Temperature Jump Methods7. Measurement of Submillisecond Protein Folding using Trp Fluorescence and Photochemical Oxidation.- Native State Hydrogen Exchange-Mass Spectrometry Methods to Probe Protein Folding and Unfolding.- Multi-Probe Equilibrium Analysis of Gradual (Un)Folding Processes.- Nmr Analysis Of Protein Folding Interaction Networks.- NMR Relaxation Dispersion Methods for the Structural and Dynamic Analysis of Quickly Interconverting, Low-Populated Conformational Sub-States.- Native State Hydrogen Exchange-Mass Spectrometry Methods to Probe Protein Folding and Unfolding.- Single-Molecule Fluorescence Spectroscopy Approaches for Probing Fast Biomolecular Dynamics and Interactions.- Theory and Analysis of Single-Molecule FRET experiments.- Mechanochemical Evolution Of Disulfide Bonds In Proteins.- Coarse-Grained Simulations of Protein Folding: Bridging Theory and Experiments.- Analysis of Molecular Dynamics Simulations of Protein Folding.- Atomistic Simulations of Thermal Unfolding.- Molecular Simulations of Intrinsically Disordered Proteins and their Binding Mechanisms.- Prediction of Folding and Unfolding Rates of Proteins with Simple Models.- Predicting and Simulating Mutational Effects on Protein Folding Kinetics.- Localization of Energetic Frustration in Proteins.- Modeling the Structure, Dynamics, and Transformations of Proteins with the UNRES Force Field.