About the Editors

Michael F. Murphy, MD, FRCP, FRCPath, FFPath, is Professor of Blood Transfusion Medicine at the University of Oxford, and Consultant Haematologist for NHS Blood and Transplant and the Department of Haematology, Oxford University Hospitals NHS Foundation Trust, Oxford, UK.

David J. Roberts, MB, ChB, DPhil, FRCPath, is Professor of Haematology at the University of Oxford, Medical Director for Pathology for NHS Blood and Transplant and also works as a Consultant Haematologist in the Department of Haematology, John Radcliffe Hospital, Oxford, UK.

Mark H. Yazer, MD is Professor of Pathology at the University of Pittsburgh, an Adjunct Professor of Clinical Immunology at the University of Southern Denmark, Odense, Denmark, and a Visiting Professor of Pathology at Tel Aviv University. His research interest is in blood use in massive bleeding.

Nancy M. Dunbar, MD is Associate Professor of Pathology and Laboratory Medicine at the Geisel School of Medicine at Dartmouth and serves as the Medical Director of the Blood Bank at Dartmouth-Hitchcock Medical Center, Lebanon, NH, USA.

List of Contributors ix

Preface to the Sixth Edition xvi

1 Introduction: Two Centuries of Progress in Transfusion Medicine 1
Walter H. (Sunny) Dzik and Michael F. Murphy

Part I Basic Principles of Immunohaematology 11

2 Essential Immunology for Transfusion Medicine 11
Gregory A. Denomme

3 Human Blood Group Systems 21
Geoff Daniels and Sean Stowell

4 Human Leukocyte Antigens 33
H. Cliff Sullivan and Caroline R. Alquist

5 Platelet and Neutrophil Antigens 47
Brian R. Curtis

6 Pretransfusion Testing and the Selection of Red Cell Products for Transfusion 64
Mark H. Yazer, Meghan Delaney and Gregory A. Denomme Copyrighted Material

Part II Complications of Transfusions 77

7 Investigation of Acute Transfusion Reactions 77
Heather Leary, Kathryn E. Webert and Nancy M. Heddle

8 Haemolytic Transfusion Reactions 91
Edwin J. Massey, Robertson D. Davenport and Richard M. Kaufman

9 Febrile and Allergic Transfusion Reactions 108
Sarah K. Harm and Mark K. Fung

10 Transfusion- Associated Circulatory Overload 120
Robert B. Klanderman and Alexander P.J. Vlaar

11 Transfusion- Related Acute Lung Injury (TRALI) 130
Alexander P.J. Vlaar and Rick Kapur

12 Purported Adverse Effects of 'Old Blood' 145
Lirong Qu and Darrell J. Triulzi

13 Transfusion- Induced Immunomodulation 154
Neil Blumberg, Majed A. Refaai and Joanna M. Heal

14 Transfusion- Associated Graft- versus- Host Disease and Microchimerism 166
Alexandra Budhai, Richard O. Francis and Beth H. Shaz

15 Posttransfusion Purpura 181
Michael F. Murphy

16 Heparin- Induced Thrombocytopenia 187
Theodore E. Warkentin and Andreas Greinacher

17 Transfusion- Transmitted Infections 206
Steven J. Drews and Roger Y. Dodd

18 Bacterial Contamination 221
Sandra Ramírez- Arcos and Mindy Goldman

19 Emerging Infections and Transfusion Safety 229
Roger Y. Dodd

Part III Practice in Blood Centres and Hospitals 238

20 Regulatory Aspects of Blood Transfusion 238
Louis M. Katz, Peter Flanagan and William G. Murphy

21 The Role of Hemovigilance in Transfusion Safety 247
Katharine A. Downes and Barbee I. Whitaker

22 Donors and Blood Collection 257
Marc Germain and Pierre Tiberghien

23 Blood Donation Testing and the Safety of the Blood Supply 269
Mindy Goldman and Steven J. Drews

24 Production and Storage of Blood Components 278
Marissa Li, Rebecca Cardigan, Stephen Thomas and Ralph Vassallo

25 Blood Transfusion in Hospitals 290
Zoe K. McQuilten, Erica M. Wood, Mark H. Yazer and Michael F. Murphy

26 Disaster Preparedness 313
Heidi Doughty, Fateha Chowdhury and Richard Rackham

27 Blood Transfusion in a Global Context 324
David J. Roberts, Imelda Bates, Stephen P. Field, Aggrey Dhabangi, Jean Pierre Allain and Meghan Delaney

Part IV Clinical Transfusion Practice 337

28 Inherited and Acquired Disorders of Haemostasis 337
Irina Chibisov, Jansen Seheult and Franklin Bontempo

29 Point- of- Care Haemostasis Testing 353
Katrina M. Morgan, Matthew D. Neal, Louis Alarcon and Christine M. Leeper

30 Transfusion in Bleeding Patients 365
Aaron S. Hess, Lynn G. Stansbury and John R. Hess

31 Transfusion in Non- Bleeding Medical and Surgical Patients 374
Killian Donovan, Akshay Shah, Stuart McKechnie and Simon J. Stanworth

32 Transfusion in Malignant Haematological Disease 385
Lise J. Estcourt and Michael F. Murphy

33 Transfusion in Benign Haematological Disease 399
Eric Salazar and Christopher A. Tormey

34 Transfusion in Patients with Haemoglobinopathies 409
Yvette C. Tanhehco and Patricia A. Shi

35 Transfusing Neonates and Infants 417
Marianne E. Yee and Cassandra D. Josephson

36 Immunodeficiency and Immunoglobulin Therapy 426
Siraj A. Misbah

Part V Patient Blood Management 441

37 Development of a Patient Blood Management Programme 441
Jonathan H. Waters

38 Perioperative Patient Blood Management 451
Martin Shao Foong Chong, Martin Rooms, Ravishankar Rao Baikady and Toby Richards

39 Restrictive Transfusion Practice and How to Implement It 465
Marie A. Hollenhorst and Lawrence Tim Goodnough

40 Using Data to Support Patient Blood Management 476
Nadia B. Hensley, Sandaruwani Abeysiri and Steven M. Frank

Part VI Cellular Therapy and Transplantation 489

41 Regulation and Accreditation in Cellular Therapy 489
Julia Wolf, Zbigniew (Ziggy) M. Szczepiorkowski and James Griffin

42 Stem Cell Collection and Therapeutic Apheresis 504
Khaled El- Ghariani and Zbigniew (Ziggy) M. Szczepiorkowski

43 Haemopoietic Stem Cell Processing and Storage 519
Akash Gupta and Ronan Foley

44 Haematopoietic Cell Transplantation 531
Richard Salisbury, Yisu Gu, Rachael Hough and Robert D. Danby

45 CAR- T Cells and Recent Advances in Clinical Cellular Immunotherapy 543
Yisu Gu, Alex Rampotas, Connor Sweeney, David J. Roberts and Ronjon Chakraverty

46 Tissue Banking 554
Akila Chandrasekar, Richard Lomas and Paul Rooney

Part VII Development of the Evidence Base for Transfusion 561

47 Observational and Interventional Trials in Transfusion Medicine 561
Alan T. Tinmouth, Dean Fergusson and Paul C. Hébert

48 Getting the Most Out of the Evidence for Transfusion Medicine and Lessons from the COVID- 19 Pandemic 576
Simon J. Stanworth, Susan J. Brunskill, Carolyn Dorée and Yulia Lin

49 A Primer on Biostatistics 590
Andrew W. Shih, Na Li, Celina Montemayor and Nancy M. Heddle

50 A Primer on Health Economics 609
Seema Kacker and Aaron A. R. Tobian

51 Scanning the Future of Transfusion Medicine 620
Jay E. Menitove, Paul M. Ness, Suzanne R. Thibodeaux and Edward L. Snyder

Index 632