Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible result

Contains key notes and implementation advice from the experts

The significance of PCR primer design in genetic diversity studies; exemplified by recent research into the genetic structure of marine species.- Enhancing cohort PASA efficiency from lessons assimilated by mutant genotyping in C. elegans.- Design of oligonucleotides for allele-specific amplification based on PCR and isothermal techniques.- Detection of rubella virus by tri-primer RT-PCR assay and genotyping by fragment RT-PCR.- Design of mismatch primers to identify and differentiate closely related (sub)species - application to the authentication of meat products.- Primer design for the analysis of closely related species - application of non-coding mtDNA and cpDNA sequences.- Designing PCR primers for the amplification-refractory mutation system.- Validation of circular RNAs by PCR.- Primer Designing for Amplifying an AT-Rich Promoter from Arabidopsis thaliana.- PLASmid TAXonomic PCR (PlasTax-PCR), a multiplex relaxase MOB typing to assort plasmids into taxonomic units.- Multiplex PCR Design for Scalable Resequencing.- Identification of gene copy number in the transgenic plants by quantitative polymerase chain reaction (qPCR).- qPrimerDB: A powerful and user-friendly database for qPCR primer design.- PCR primer design for the rapidly evolving SARS-CoV-2 genome.- Universal primers for detection of novel plant capsid-less viruses: Papaya umbra-like viruses as example.- A guide to using FASTPCR software for PCR, in silico PCR, and oligonucleotide analysis.- Pyrosequencing Primer Design for Forensic Biology Applications.-Phosphate¿ ¿methylated¿ ¿oligonucleotides¿ ¿as¿ ¿ä ¿novel¿ ¿primer¿ ¿for¿ ¿PCR¿ ¿and¿ ¿RT-PCR¿.