Includes cutting-edge methods and protocols for the study of organoids

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment.- Clinically-Amendable Defined and Rapid Induction of Human Brain Organoids from Induced Pluripotent Stem Cells.- Organoid Assay of Human Cancer Stem Cells.- Construction of Thymus Organoids from Decellularized Thymus Scaffolds.- Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres.- Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells.- Organoid Culture of Lingual Epithelial Cells in a Three-Dimensional Matrix.- Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension.- Efficient Culture of Intestinal Organoids with Blebbistatin.- Isolation and Culture of Adult Intestinal, Gastric, and Liver Organoids for Cre-Recombinase-Mediated Gene Deletion.- The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine.- New Trends and Perspectives in theFunction of Non-Neuronal Acetylcholine in Crypt-Villus Organoids in Mice.- Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System.- Murine Colonic Organoid Culture System and Downstream Assay Applications.- Intestinal Organoids as a Novel Tool to Study Microbes-Epithelium Interactions.- The Isolation, Culture, and Propagation of Murine Intestinal Enteroids for the Study of Dietary Lipid Metabolism.- Oncogenic Transformation of Human-Derived Gastric Organoids.- Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation.- Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections.- Study Bacterial-Host Interactions Using Intestinal Organoids.- Disaggregation and Reaggregation of Zebrafish Retinal Cells for the Analysis of Neuronal Layering.- Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo.- Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells.- Use of a Super-Hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-Organoids.- Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly.- Cell Microencapsulation in Polyethylene Glycol Hydrogel Microspheres Using Electrohydrodynamic Spraying.- Gastrointestinal Epithelial Organoid Cultures from Post-Surgical Tissues.- Drug Sensitivity Assays of Human Cancer Organoid Cultures.