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Oligodendrocytes
Methods and Protocols
Buch von Linde Kegel (u. a.)
Sprache: Englisch

174,95 €*

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Beschreibung
This volume looks at the study of oligodendrocytes through in vitro and in vivo techniques, multiple model organisms, using approaches that bridge scales from molecular through system. Chapters in this book cover topics such as fundamental molecular analyses of oligodendrocytes and myelin; in vitro, ex vivo, and in vivo molecular-cellular-electrophysiology-based techniques; oligodendrocyte formation, homeostasis, and disruption in zebrafish and Xenopus; and parallel system-level imaging of animal and human models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Oligodendrocytes: Methods and Protocols is a valuable reference guide that highlights the expansive and fast-paced nature of research into oligodendrocyte biology underlying health and function.
This volume looks at the study of oligodendrocytes through in vitro and in vivo techniques, multiple model organisms, using approaches that bridge scales from molecular through system. Chapters in this book cover topics such as fundamental molecular analyses of oligodendrocytes and myelin; in vitro, ex vivo, and in vivo molecular-cellular-electrophysiology-based techniques; oligodendrocyte formation, homeostasis, and disruption in zebrafish and Xenopus; and parallel system-level imaging of animal and human models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Oligodendrocytes: Methods and Protocols is a valuable reference guide that highlights the expansive and fast-paced nature of research into oligodendrocyte biology underlying health and function.
Zusammenfassung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Inhaltsverzeichnis
Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous System.- Isolation and Preparation of Cells from Focal Remyelinating Central Nervous System Lesion sfor RNA Sequencing.- Myelin: Methods for Purification and Proteome Analysis.- In Vitro Generation and Electrophysiological Characterization of OPCc and Oligodendrocytes from Human Pluripotent Stem Cells.- Isolation and Culture of Oligodendrocytes.- A Neuron-Free Microfiber Assay to Assess Myelin Sheath Formation.- Oligodendrocyte-Neuron Myelinating Co-Culture.- Teasing of Ventral Spinal Cord White Matter Fibres for the Analysis of Central Nervous System Nodes of Ranvier.- Whole-Cell Patch Clamp Recordings from Oligodendrocyte Lineage Cells in Brain Slices.- Ex Vivo Slice Cultures to Study Myelination, Demyelination, and Remyelination in Mouse Brain and Spinal Cord.- Forward Genetic Screen using Zebrafish to Identify New Genes Involved in Myelination.- Manipulating Neuronal Activity in the Developing Zebrafish Spinal Cord to Investigate Adaptive Myelination.- A Drug Inducible Transgenic Zebrafish Model for Myelinating Glial Cell Ablation.- Conditional Demyelination and Remyelination in a Transgenic Xenopus laevis.- Conditional Mutagenesis in Oligodendrocyte Lineage Cells.- Recent Advances in Live Imaging of Cells of the Oligodendrocyte Lineage.- The DTA Mouse Model for Oligodentrocyte Ablation and CNS Demyelination.- Human Glial Chimeric Mice to Define the Role of Glial Pathology in Human Disease.- A Method to Detect Cytochrome c Oxidase Activity and Mitochondrial Proteins in Oligodencrocytes.- Transmission Electron Microscopy of Oligodendrocytes and Myelin.- Toxin-Based Models to Investigate Demyelination and Remyelination.- Magnetic Resonance techniques for Imaging White Matter.
Details
Erscheinungsjahr: 2019
Fachbereich: Allgemeines
Genre: Biologie
Rubrik: Naturwissenschaften & Technik
Thema: Lexika
Medium: Buch
Seiten: 424
Reihe: Methods in Molecular Biology
Inhalt: xii
411 S.
10 s/w Illustr.
55 farbige Illustr.
411 p. 65 illus.
55 illus. in color.
ISBN-13: 9781493990702
ISBN-10: 1493990705
Sprache: Englisch
Herstellernummer: 978-1-4939-9070-2
Ausstattung / Beilage: HC runder Rücken kaschiert
Einband: Gebunden
Redaktion: Kegel, Linde
Lyons, David A.
Herausgeber: David A Lyons/Linde Kegel
Auflage: 1st ed. 2019
Hersteller: Springer US
Springer New York
Methods in Molecular Biology
Maße: 260 x 183 x 29 mm
Von/Mit: Linde Kegel (u. a.)
Erscheinungsdatum: 01.03.2019
Gewicht: 0,99 kg
preigu-id: 114825984
Zusammenfassung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Inhaltsverzeichnis
Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous System.- Isolation and Preparation of Cells from Focal Remyelinating Central Nervous System Lesion sfor RNA Sequencing.- Myelin: Methods for Purification and Proteome Analysis.- In Vitro Generation and Electrophysiological Characterization of OPCc and Oligodendrocytes from Human Pluripotent Stem Cells.- Isolation and Culture of Oligodendrocytes.- A Neuron-Free Microfiber Assay to Assess Myelin Sheath Formation.- Oligodendrocyte-Neuron Myelinating Co-Culture.- Teasing of Ventral Spinal Cord White Matter Fibres for the Analysis of Central Nervous System Nodes of Ranvier.- Whole-Cell Patch Clamp Recordings from Oligodendrocyte Lineage Cells in Brain Slices.- Ex Vivo Slice Cultures to Study Myelination, Demyelination, and Remyelination in Mouse Brain and Spinal Cord.- Forward Genetic Screen using Zebrafish to Identify New Genes Involved in Myelination.- Manipulating Neuronal Activity in the Developing Zebrafish Spinal Cord to Investigate Adaptive Myelination.- A Drug Inducible Transgenic Zebrafish Model for Myelinating Glial Cell Ablation.- Conditional Demyelination and Remyelination in a Transgenic Xenopus laevis.- Conditional Mutagenesis in Oligodendrocyte Lineage Cells.- Recent Advances in Live Imaging of Cells of the Oligodendrocyte Lineage.- The DTA Mouse Model for Oligodentrocyte Ablation and CNS Demyelination.- Human Glial Chimeric Mice to Define the Role of Glial Pathology in Human Disease.- A Method to Detect Cytochrome c Oxidase Activity and Mitochondrial Proteins in Oligodencrocytes.- Transmission Electron Microscopy of Oligodendrocytes and Myelin.- Toxin-Based Models to Investigate Demyelination and Remyelination.- Magnetic Resonance techniques for Imaging White Matter.
Details
Erscheinungsjahr: 2019
Fachbereich: Allgemeines
Genre: Biologie
Rubrik: Naturwissenschaften & Technik
Thema: Lexika
Medium: Buch
Seiten: 424
Reihe: Methods in Molecular Biology
Inhalt: xii
411 S.
10 s/w Illustr.
55 farbige Illustr.
411 p. 65 illus.
55 illus. in color.
ISBN-13: 9781493990702
ISBN-10: 1493990705
Sprache: Englisch
Herstellernummer: 978-1-4939-9070-2
Ausstattung / Beilage: HC runder Rücken kaschiert
Einband: Gebunden
Redaktion: Kegel, Linde
Lyons, David A.
Herausgeber: David A Lyons/Linde Kegel
Auflage: 1st ed. 2019
Hersteller: Springer US
Springer New York
Methods in Molecular Biology
Maße: 260 x 183 x 29 mm
Von/Mit: Linde Kegel (u. a.)
Erscheinungsdatum: 01.03.2019
Gewicht: 0,99 kg
preigu-id: 114825984
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