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Authoritative and thorough, Nucleic Acid Crystallography: Methods and Protocols presents protocols that are aimed at both researchers and students who are interested in the structural biology of DNA or RNA, alone or in complex with proteins or ligands.
Authoritative and thorough, Nucleic Acid Crystallography: Methods and Protocols presents protocols that are aimed at both researchers and students who are interested in the structural biology of DNA or RNA, alone or in complex with proteins or ligands.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Perspectives and Pitfalls in Nucleic Acids Crystallography.- Preparation of Short 5'-Triphosphorylated Oligoribonucleotides for Crystallographic and Biochemical Studies.- Preparation and Crystallization of Riboswitches.- In Vitro/In Vivo Production of tRNA for X-Ray Studies.- Polyacrylamide Gel Electrophoresis for Purification of Large Amounts of RNA.- Use of the U1A Protein to Facilitate Crystallization and Structure Determination of Large RNAs.- Fab Chaperone-Assisted RNA Crystallography (Fab CARC).- Generating Crystallographic Models of DNA Dodecamers from Structures of RNase H: DNA Complexes.- Isothermal Titration Calorimetry: Assisted Crystallization of RNA-Ligand Complexes.- Crystallographic Data and Model Quality.- Advanced Crystallographic Data Collection Protocols for Experimental Phasing.- Nucleic Acid Crystallography via Direct Selenium Derivatization: RNAs Modified with Se-Nucleobases.- Practical Radiation Damage Induced Phasing.- Soaking Hexammine Cations into RNA Crystals to Obtain Derivatives for Phasing Diffraction Data.- Using Molecular Replacement Phasing to Study the Structure and Function of RNA.- Helical Symmetry of Nucleic Acids: Obstacle or Help in Structure Solution?.- RNA Structure Refinement Using the ERRASER-Phenix Pipeline.- Neutron Nucleic Acid Crystallography.- Reconstitution of Functionally Active Thermus thermophilus 30S Ribosomal Subunit from Ribosomal 16S RNA and Ribosomal Proteins.- Crystallographic Studies of the Ribosomal A-Site Molecular Switches by Using Model RNA Oligomers.- Structure of the HCV Internal Ribosome Entry Site Subdomain IIa RNA in Complex with a Viral Translation Inhibitor.- Anions in Nucleic Acid Crystallography.
Erscheinungsjahr: | 2015 |
---|---|
Fachbereich: | Organische Chemie |
Genre: | Chemie |
Rubrik: | Naturwissenschaften & Technik |
Medium: | Buch |
Seiten: | 368 |
Reihe: | Methods in Molecular Biology |
Inhalt: |
xi
356 S. 18 s/w Illustr. 98 farbige Illustr. 356 p. 116 illus. 98 illus. in color. |
ISBN-13: | 9781493927623 |
ISBN-10: | 1493927620 |
Sprache: | Englisch |
Herstellernummer: | 978-1-4939-2762-3 |
Ausstattung / Beilage: | HC runder Rücken kaschiert |
Einband: | Gebunden |
Redaktion: | Ennifar, Eric |
Herausgeber: | Eric Ennifar |
Auflage: | 1st ed. 2015 |
Hersteller: |
Springer US
Springer New York Methods in Molecular Biology |
Maße: | 260 x 183 x 25 mm |
Von/Mit: | Eric Ennifar |
Erscheinungsdatum: | 31.07.2015 |
Gewicht: | 0,972 kg |
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Perspectives and Pitfalls in Nucleic Acids Crystallography.- Preparation of Short 5'-Triphosphorylated Oligoribonucleotides for Crystallographic and Biochemical Studies.- Preparation and Crystallization of Riboswitches.- In Vitro/In Vivo Production of tRNA for X-Ray Studies.- Polyacrylamide Gel Electrophoresis for Purification of Large Amounts of RNA.- Use of the U1A Protein to Facilitate Crystallization and Structure Determination of Large RNAs.- Fab Chaperone-Assisted RNA Crystallography (Fab CARC).- Generating Crystallographic Models of DNA Dodecamers from Structures of RNase H: DNA Complexes.- Isothermal Titration Calorimetry: Assisted Crystallization of RNA-Ligand Complexes.- Crystallographic Data and Model Quality.- Advanced Crystallographic Data Collection Protocols for Experimental Phasing.- Nucleic Acid Crystallography via Direct Selenium Derivatization: RNAs Modified with Se-Nucleobases.- Practical Radiation Damage Induced Phasing.- Soaking Hexammine Cations into RNA Crystals to Obtain Derivatives for Phasing Diffraction Data.- Using Molecular Replacement Phasing to Study the Structure and Function of RNA.- Helical Symmetry of Nucleic Acids: Obstacle or Help in Structure Solution?.- RNA Structure Refinement Using the ERRASER-Phenix Pipeline.- Neutron Nucleic Acid Crystallography.- Reconstitution of Functionally Active Thermus thermophilus 30S Ribosomal Subunit from Ribosomal 16S RNA and Ribosomal Proteins.- Crystallographic Studies of the Ribosomal A-Site Molecular Switches by Using Model RNA Oligomers.- Structure of the HCV Internal Ribosome Entry Site Subdomain IIa RNA in Complex with a Viral Translation Inhibitor.- Anions in Nucleic Acid Crystallography.
Erscheinungsjahr: | 2015 |
---|---|
Fachbereich: | Organische Chemie |
Genre: | Chemie |
Rubrik: | Naturwissenschaften & Technik |
Medium: | Buch |
Seiten: | 368 |
Reihe: | Methods in Molecular Biology |
Inhalt: |
xi
356 S. 18 s/w Illustr. 98 farbige Illustr. 356 p. 116 illus. 98 illus. in color. |
ISBN-13: | 9781493927623 |
ISBN-10: | 1493927620 |
Sprache: | Englisch |
Herstellernummer: | 978-1-4939-2762-3 |
Ausstattung / Beilage: | HC runder Rücken kaschiert |
Einband: | Gebunden |
Redaktion: | Ennifar, Eric |
Herausgeber: | Eric Ennifar |
Auflage: | 1st ed. 2015 |
Hersteller: |
Springer US
Springer New York Methods in Molecular Biology |
Maße: | 260 x 183 x 25 mm |
Von/Mit: | Eric Ennifar |
Erscheinungsdatum: | 31.07.2015 |
Gewicht: | 0,972 kg |