Shohreh Amini: Neuronal Cell Culture

Cover: 9781493960644 | Neuronal Cell Culture | Methods and Protocols | Shohreh Amini (u. a.)

Rückseite: 9781493960644 | Neuronal Cell Culture | Methods and Protocols | Shohreh Amini (u. a.)

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Sprache: Englisch

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Beschreibung

In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved. Starting with a general overview of the neuronal culturing principles that are described, this detailed volume covers cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and easy to use, Neuronal Cell Culture: Methods and Protocols will be of interest to scientists at all levels developing cell culture models for neuroscientific studies.

Zusammenfassung

Covers the latest aspects of the culture of neural cells

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Inhaltsverzeichnis

General Overview of Neuronal Cell Culture.- Considerations for the Use of
SH-SY5Y
Neuroblastoma Cells in Neurobiology.- Cultured Cell Line Models of Neuronal Differentiation: NT2, PC12.- Murine Teratocarcinoma-Derived Neuronal Cultures.- Isolation and Propagation of Primary Human and Rodent Embryonic Neural Progenitor Cells and Cortical Neurons.- Mouse Enteric Neuronal Cell Culture.- Preparation of Neural Stem Cells and Progenitors: Neuronal Production and Grafting Applications.- Derivation of Neuronal Cells from Fetal Normal Human Astrocyte (NHA).- Slice Culture Modeling of Central Nervous System (CNS) Viral Infection.- Neurospheres and Glial Cell Cultures: Immunocytochemistry for Cell Phenotyping.- Transfection of Neuronal Cultures.- Lentiviral Transduction of Neuronal Cells.- Compartmentalized Neuronal Cultures.- Quantitative Assessment of Neurite Outgrowth Over Growth Promoting or Inhibitory Substrates.

Details

Erscheinungsjahr:	2016
Fachbereich:	Therapie
Genre:	Importe , Medizin
Rubrik:	Wissenschaften
Medium:	Taschenbuch
Inhalt:	x 165 S. 7 s/w Illustr. 31 farbige Illustr. 165 p. 38 illus. 31 illus. in color.
ISBN-13:	9781493960644
ISBN-10:	1493960644
Sprache:	Englisch
Einband:	Kartoniert / Broschiert
Redaktion:	Amini, Shohreh White, Martyn K.
Herausgeber:	Shohreh Amini/Martyn K White
Auflage:	Softcover reprint of the original 1st edition 2013
Hersteller:	Humana Humana Press Inc.
Verantwortliche Person für die EU:	Humana Press in Springer Science + Business Media, Heidelberger Platz 3, D-14197 Berlin, juergen.hartmann@springer.com
Maße:	254 x 178 x 10 mm
Von/Mit:	Shohreh Amini (u. a.)
Erscheinungsdatum:	23.08.2016
Gewicht:	0,344 kg