Includes cutting-edge methods and protocols for the manipulation of mitochondrial function

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

1. DQAsomes as the Prototype of Mitochondria-targeted Pharmaceutical Nanocarriers: Preparation, Characterization, and Use

Volkmar Weissig

2. Predicting Mitochondrial Targeting by Small Molecule Xenobiotics Within Living Cells Using QSAR Models

Richard W. Horobin

3. Targeting Mitochondria with Small Molecules: The Preparation of MitoB and MitoP as Exomarkers of Mitochondrial Hydrogen Peroxide

Andrew G. Cairns, Stephen J. McQuaker, Michael P. Murphy, and Richard C. Hartley

4. Synthesis of Triphenylphosphonium Phospholipid Conjugates for the Preparation of Mitochondriotropic Liposomes

Parul Benein, Mohammed Almuteri, Ahmed Mehanna, and Gerard G.M. D'Souza

5. Synthesis and Evaluation of 18F-Labeled Fluoroalkyl Triphenylphosphonium Salts as Mitochondrial Voltage Sensors in PET Myocardial Imaging

Dong-Yeon Kim and Jung-Joon Min

6. Bridging the Gap Between Nature and Antioxidant Setbacks: Delivering Caffeic Acid to Mitochondria

José Teixeira, Pedro Soares, Sofia Benfeito, Michael P Murphy, Paulo J. Oliveira, and Fernanda Borges

7. Mitochondriotropic Nano-Emulsified Genistein-Loaded Vehicles for Cancer Therapy

Jimmy Pham, Oliver Grundmann, and Tamer Elbayoumi

8. Formulation and Optimization of Mitochondria-Targeted Polymeric Nanoparticles

Sean Marrache, Rakesh K. Pathak, and Shanta Dhar

9. Ex Vivo Generation of Functional Immune Cells by Mitochondria-Targeted Photosensitization of Cancer Cells

Sean Marrache, Smanla Tundup, Donald A. Harn, and Shanta Dhar

10. Targeting the Mitochondrial Genome Via a Dual Function MITO-Porter: Evaluation of mtDNA-Levels and Mitochondrial Function

Yuma Yamada and Hideyoshi Harashima

11. Mitochondrial, Acidic, and Cytosolic pHs Determination by 31P NMR Spectroscopy: Design of New Sensitive Targeted pH Probes

Marcel Culcasi, Sophie Thétiot-Laurent, Ariane Atteia, and Sylvia Pietri

12. Methodology for Use of Mitochondria-Targeted Cations in the Field of Oxidative Stress-Related Research

Mikhail Y. Vyssokikh, Yury N. Antonenko, Konstantin G. Lyamzaev, Tatyana I. Rokitskaya, and Vladimir P. Skulachev

13. Synthesis and Testing of Novel Isomeric Mitochondriotropic Derivatives of Resveratrol and Quercetin

Lucia Biasutto, Andrea Mattarei, and Cristina Paradisi

14. Evaluation of Respiration of Mitochondria in Cancer Cells Exposed to Mitochondria-Targeted Agents

Katarina Kluckova, Lan-Feng Dong, Martina Bajzikova, Jakub Rohlena, and Jiri Neuzil

15. The Effect of Mitochondrially Targeted Anti-Cancer Agents on Mitochondrial (Super)Complexes

Magdalena Vondrusova, Ayanachew Bezawork-Geleta, Karishma Sachaphibulkij, Jaroslav Truksa, and Jiri Neuzil

16. Mitochondrial Targeting of Recombinant RNA

Ilya Dovydenko, Anne-Marie Heckel, Yann Tonin, Ali Gowher, Alya Venyaminova, Ivan Tarassov, and Nina Entelis

17. Mitochondrial Targeting of Catalytic RNAs

Daria Mileshina, Adnan Khan Niazi, Eliza Wyszko, Maciej Szymanski, Romain Val, Clarisse Valentin, Jan Barciszewski, and André Dietrich

18. Allotopic Expression of ATP6 in the Mouse as a Transgenic Model of Mitochondrial Disease

David A. Dunn and Carl A. Pinkert

19. Analysis of Pollutant-Induced Changes in Mitochondrial DNA Methylation

Hyang-Min Byun and Timothy M. Barrow

20. ETS and DEAS Studies of the Reduction of Xenobiotics in Mitochondrial Intermembrane Space

Stanislav A. Pshenichnyuk and Alberto Modelli

21. Analysis of Mitochondrial Morphology and Function Under Conditions of Mitofusin 2 Deficiency

Juan Pablo Muñoz and Antonio Zorzano

22. Yeast as a Tool to Study Mitochondrial Retrograde Pathway En Route to Cell Stress Response

MaSa Zdralevic, Nicoletta Guaragnella and Sergio Giannattasio

23. Cell Energy Budget Platform for Assessment of Cell Metabolism

Dmitri B. Papkovsky and Alexander V. Zhdanov

24. Induced Pluripotent Stem Cells (iPSCs) for Modeling Mitochondrial DNA Disorders

Alessandro Prigione

25. Cytoplasmic Transfer Methods for Studying the Segregation of Mitochondrial DNA in Mice

Thomas Kolbe, Ralf Steinborn, and Joerg P. Burgstaller

26. In Vivo Visualization and Quantification of Mitochondrial Morphology in C. elegans

Reuben L. Smith, Winnok H. De Vos, Richard de Boer, Erik M.M. Manders, and Hans van der Spek

27. Mitochondrial DNA as a Biosensor of UV Exposure in Human Skin

Amy Bowman and Mark A. Birch-Machin

28. Assessment of Short- and Medium-Chain Fatty Acids on Mitochondrial Function in Severe Inflammation

Matthias Hecker, Natascha Sommer, and Konstantin Mayer

29. Atomic Force Microscopy-Based Shape Analysis of Heart Mitochondria

Gi-Ja Lee and Hun-Kuk Park

30. Enzymatic Assays for Probing Mitochondrial Apoptosis

Zhenyu Wang, Claire Nicolas, Rodolphe Fischmeister, and Catherine Brenner

31. A Cybrid Cell Model for the Assessment of the Link Between Mitochondrial Deficits and Sporadic Parkinson's Disease

Daniela M. Arduíno, A. Raquel Esteves, Russell H. Swerdlow, and Sandra M. Cardoso

32. Analysis of Mitochondrial Network by Imaging: Proof of Technique in Schizophrenia

Dorit Ben-Shachar, Edith Suss-Toby, and Odile Robicsek