Microfluidics for Single-Cell Analysis | Buch

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Cover: 9789813297289 | Microfluidics for Single-Cell Analysis | Jin-Ming Lin | Buch | vii

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Sprache: Englisch

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Beschreibung

This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor¿s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.

Über den Autor

Zusammenfassung

Summarizes the various microfluidic-based single cell analysis methods

Reviews the state-of-the-art techniques showcasing the pros and cons of each method in details

Offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in reality

Inhaltsverzeichnis

Advances of single-cell analysis on microfluidics.- Microfluidic technology for single-cell capture and isolation.- Single cell culture and analysis on microfluidics.- Droplet microfluidic technology for single cell manipulation.- Droplet-based microfluidics for single cell encapsulation and analysis.- Microfluidics for Single-Cell Genomics.- Microfluidics-mass spectrometry combination systems for single cell analysis.- Micro/nano fluidics-enabled single-cell biochemical analysis.- Microfluidic chip based Live Single-Cell Probes.

Details

Erscheinungsjahr:	2019
Fachbereich:	Technik allgemein
Genre:	Technik
Rubrik:	Naturwissenschaften & Technik
Medium:	Buch
Seiten:	272
Reihe:	Integrated Analytical Systems
Inhalt:	vii 261 S. 7 s/w Illustr. 99 farbige Illustr. 261 p. 106 illus. 99 illus. in color.
ISBN-13:	9789813297289
ISBN-10:	981329728X
Sprache:	Englisch
Ausstattung / Beilage:	HC runder Rücken kaschiert
Einband:	Gebunden
Redaktion:	Lin, Jin-Ming
Herausgeber:	Jin-Ming Lin
Auflage:	1st ed. 2019
Hersteller:	Springer Singapore Springer Nature Singapore Integrated Analytical Systems
Maße:	241 x 160 x 21 mm
Von/Mit:	Jin-Ming Lin
Erscheinungsdatum:	06.09.2019
Gewicht:	0,576 kg

preigu-id: 116933333