Microcalorimetry of Biological Molecules

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Sprache: Englisch

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Beschreibung

This volume provides methods on microcalorimetry approaches to investigate complex biological molecular systems. Chapters guide readers through Differential Scanning Calorimetry (DSC), Isothermal Titration Calorimetry (ITC), and advanced data processing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Microcalorimetry of Biological Molecules: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Zusammenfassung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains keynotes and implementation advice from the experts

Inhaltsverzeichnis

The Contribution of Differential Scanning Calorimetry for the Study of Peptide/Lipid Interactions.- Protocols of IATC, DSC, and PPC: The Multi-State Structural Transition of Cytochrome c.- Value of DSC in Characterization and Optimization of Protein Stability.- Plasmatic Signature of Disease by Differential Scanning Calorimetry (DSC).- Intrinsic Thermodynamics of Protein - Ligand Binding by Isothermal Titration Calorimetry as Aid to Drug Design.- Isothermal Titration Calorimetry Measurements of Riboswitch-Ligand Interactions.- ITC Studies of Ribosome/Antibiotics Interactions.- High Quality Data of Protein/Peptide Interaction by Isothermal Titration Calorimetry.- ITC Measurement for High Affinity Aptamers Binding to their Target Proteins.- Thermodynamics of Molecular Machines using Incremental ITC.- Measuring the Metabolic Activity of Mature Mycobacteria Biofilms using Isothermal Microcalorimetry.- Characterization of Microtubule-Associated Proteins (MAPs) and Tubulin Interactions by Isothermal Titration Calorimetry (ITC).- Analysis of Isothermal Titration Calorimetry Data for Complex Interactions using I2CITC.- Tinkering with Binding Polynomials in Isothermal Titration Calorimetry.- Use of ITC and the Software AFFINImeter for the Quantification of Anticoagulant Pentasaccharide in Low Molecular Weight Heparine.- Thermodynamic and Kinetic Analysis of Isothermal Titration Calorimetry Experiments by using kinITC in AFFINImeter.- Enzyme Kinetics Determined by Single-Injection Isothermal Titration Calorimetry.- Characterization of Enzymatic Reactions using ITC.

Erscheinungsjahr:	2019
Fachbereich:	Biophysik
Genre:	Biologie , Importe
Rubrik:	Naturwissenschaften & Technik
Medium:	Buch
Inhalt:	xi 268 S. 20 s/w Illustr. 40 farbige Illustr. 268 p. 60 illus. 40 illus. in color.
ISBN-13:	9781493991785
ISBN-10:	1493991787
Sprache:	Englisch
Herstellernummer:	978-1-4939-9178-5
Einband:	Gebunden
Autor:	Ennifar, Eric
Redaktion:	Ennifar, Eric
Herausgeber:	Eric Ennifar
Auflage:	1st edition 2019
Hersteller:	Springer US Springer New York
Verantwortliche Person für die EU:	Humana Press in Springer Science + Business Media, Heidelberger Platz 3, D-14197 Berlin, juergen.hartmann@springer.com
Maße:	260 x 183 x 20 mm
Von/Mit:	Eric Ennifar
Erscheinungsdatum:	01.04.2019
Gewicht:	0,792 kg

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