Methicillin-Resistant Staphylococcus Aureu...

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Beschreibung

This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens.

Zusammenfassung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Inhaltsverzeichnis

Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections.- Rapid Methods for Detection of MRSA in Clinical Specimens.- Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant Staphylococcus aureus.- Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA.- Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Strains.- Spa Genotyping of Staphylococcus aureus Isolates.- Multilocus Sequence Typing of Staphylococcus aureus.- Genetic Manipulations of Staphylococcal Chromosomal DNA.- Genetic Manipulation of MRSA using CRISPR/Cas9 Technology.- RNA-seq Identification of Target Genes Mediated by Regulators of Staphylococcus aureus.- Application of Two-Dimensional Difference Gel Electrophoresis in Identification of Factors Responsible for Virulence of Staphylococcus aureus.- Identification of Virulence Determinants during Host-Pathogen Interaction using Tn-Seq Technology.- Metabolomic Profiling of Staphylococcus aureus.- Determining Impact of Growth Phases on Capacity of Staphylococcus aureus to Adhere to and Invade Host Cells.- Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections using Noninvasive Optical Imaging.- Methicillin-Resistant Staphylococcus aureus Infection and Treatment Options.- Animal Models for Drug Development for MRSA.

Details

Erscheinungsjahr:	2020
Fachbereich:	Allgemeines
Genre:	Biologie , Importe
Rubrik:	Naturwissenschaften & Technik
Thema:	Lexika
Medium:	Taschenbuch
Inhalt:	xi 268 S. 8 s/w Illustr. 27 farbige Illustr. 268 p. 35 illus. 27 illus. in color.
ISBN-13:	9781493998517
ISBN-10:	149399851X
Sprache:	Englisch
Einband:	Kartoniert / Broschiert
Redaktion:	Ji, Yinduo
Herausgeber:	Yinduo Ji
Auflage:	Third Edition 2020
Hersteller:	Springer US Springer New York
Verantwortliche Person für die EU:	Springer Verlag GmbH, Tiergartenstr. 17, D-69121 Heidelberg, juergen.hartmann@springer.com
Maße:	254 x 178 x 16 mm
Von/Mit:	Yinduo Ji
Erscheinungsdatum:	16.09.2020
Gewicht:	0,532 kg