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Beschreibung
This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens.
This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens.
Zusammenfassung
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Inhaltsverzeichnis
Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections.- Rapid Methods for Detection of MRSA in Clinical Specimens.- Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant Staphylococcus aureus.- Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA.- Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Strains.- Spa Genotyping of Staphylococcus aureus Isolates.- Multilocus Sequence Typing of Staphylococcus aureus.- Genetic Manipulations of Staphylococcal Chromosomal DNA.- Genetic Manipulation of MRSA using CRISPR/Cas9 Technology.- RNA-seq Identification of Target Genes Mediated by Regulators of Staphylococcus aureus.- Application of Two-Dimensional Difference Gel Electrophoresis in Identification of Factors Responsible for Virulence of Staphylococcus aureus.- Identification of Virulence Determinants during Host-Pathogen Interaction using Tn-Seq Technology.- Metabolomic Profiling of Staphylococcus aureus.- Determining Impact of Growth Phases on Capacity of Staphylococcus aureus to Adhere to and Invade Host Cells.- Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections using Noninvasive Optical Imaging.- Methicillin-Resistant Staphylococcus aureus Infection and Treatment Options.- Animal Models for Drug Development for MRSA.
Details
Erscheinungsjahr: | 2020 |
---|---|
Fachbereich: | Allgemeines |
Genre: | Biologie, Importe |
Rubrik: | Naturwissenschaften & Technik |
Thema: | Lexika |
Medium: | Taschenbuch |
Inhalt: |
xi
268 S. 8 s/w Illustr. 27 farbige Illustr. 268 p. 35 illus. 27 illus. in color. |
ISBN-13: | 9781493998517 |
ISBN-10: | 149399851X |
Sprache: | Englisch |
Einband: | Kartoniert / Broschiert |
Redaktion: | Ji, Yinduo |
Herausgeber: | Yinduo Ji |
Auflage: | Third Edition 2020 |
Hersteller: |
Springer US
Springer New York |
Verantwortliche Person für die EU: | Springer Verlag GmbH, Tiergartenstr. 17, D-69121 Heidelberg, juergen.hartmann@springer.com |
Maße: | 254 x 178 x 16 mm |
Von/Mit: | Yinduo Ji |
Erscheinungsdatum: | 16.09.2020 |
Gewicht: | 0,532 kg |
Zusammenfassung
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Inhaltsverzeichnis
Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections.- Rapid Methods for Detection of MRSA in Clinical Specimens.- Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant Staphylococcus aureus.- Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA.- Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Strains.- Spa Genotyping of Staphylococcus aureus Isolates.- Multilocus Sequence Typing of Staphylococcus aureus.- Genetic Manipulations of Staphylococcal Chromosomal DNA.- Genetic Manipulation of MRSA using CRISPR/Cas9 Technology.- RNA-seq Identification of Target Genes Mediated by Regulators of Staphylococcus aureus.- Application of Two-Dimensional Difference Gel Electrophoresis in Identification of Factors Responsible for Virulence of Staphylococcus aureus.- Identification of Virulence Determinants during Host-Pathogen Interaction using Tn-Seq Technology.- Metabolomic Profiling of Staphylococcus aureus.- Determining Impact of Growth Phases on Capacity of Staphylococcus aureus to Adhere to and Invade Host Cells.- Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections using Noninvasive Optical Imaging.- Methicillin-Resistant Staphylococcus aureus Infection and Treatment Options.- Animal Models for Drug Development for MRSA.
Details
Erscheinungsjahr: | 2020 |
---|---|
Fachbereich: | Allgemeines |
Genre: | Biologie, Importe |
Rubrik: | Naturwissenschaften & Technik |
Thema: | Lexika |
Medium: | Taschenbuch |
Inhalt: |
xi
268 S. 8 s/w Illustr. 27 farbige Illustr. 268 p. 35 illus. 27 illus. in color. |
ISBN-13: | 9781493998517 |
ISBN-10: | 149399851X |
Sprache: | Englisch |
Einband: | Kartoniert / Broschiert |
Redaktion: | Ji, Yinduo |
Herausgeber: | Yinduo Ji |
Auflage: | Third Edition 2020 |
Hersteller: |
Springer US
Springer New York |
Verantwortliche Person für die EU: | Springer Verlag GmbH, Tiergartenstr. 17, D-69121 Heidelberg, juergen.hartmann@springer.com |
Maße: | 254 x 178 x 16 mm |
Von/Mit: | Yinduo Ji |
Erscheinungsdatum: | 16.09.2020 |
Gewicht: | 0,532 kg |
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