Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts

1. Overview and Future Directions

Yannick J. Bomble and Michael E. Himmel

2. Genetics of Unstudied Thermophiles for Industry

Daehwan Chung, Nicholas S. Sarai, Michael E. Himmel, and Yannick J. Bomble

3. Methods for Metabolic Engineering of Thermoanaerobacterium saccharolyticum

4. Methods for Metabolic Engineering of a Filamentous Trichoderma reesei

Yat-Chen Chou, Arjun Singh, Qi Xu, Michael E. Himmel, and Min Zhang

5. Methods for Algal Protein Isolation and Proteome Analysis

Eric P. Knoshaug, Alida T. Gerritsen, Calvin A. Henard, and Michael T. Guarnieri

6. An Improved Leaf Protoplast System for Highly Efficient Transient Expression in Switchgrass (Panicum virgatum L.)

Chien-Yuan Lin, Hui Wei, Bryon S. Donohoe, Chandra L. Tucker, Melvin P. Tucker, and Michael E. Himmel

7. Characterizing Intracellular Proteomes for Microbes: An Experimental Approach Using Label-Free Protein Quantitation

Paul E. Abraham and Robert L. Hettich

8. Bacterial Differential Expression Analysis Methods

Sagar Utturkar, Asela Dassanayake, Shilpa Nagaraju, and Steven D. Brown

9. Measuring Biomass-Derived Products in Biological Conversion and Metabolic Process

Chang Geun Yoo, Yunqiao Pu, and Arthur J. Ragauskas

10. Crystallography of Metabolic Enzymes

Markus Alahuhta, Michael E. Himmel, and Vladimir Lunin

11. Measuring Metabolic Enzyme Performance

Amanda Williams-Rhaesa and Michael W.W. Adams

12. Gene Editing Technologies for Biofuel Production in Thermophilic Microbes

Sharon Smolinski, Emily Freed, and Carrie Eckert

13. Software and Methods for Computational Flux Balance Analysis

Peter C. St. John and Yannick J. Bomble

14. Dynamic Flux Analysis: An Experimental Approach of Fluxomics

Wei Xiong, Huaiguang Jiang, and PinChing Maness

15. Network Modeling of Complex Data Sets

Piet Jones, Deborah Weighill, Manesh Shah, Sharlee Climer, Jeremy Schmutz, Avinash Sreedasyam, Gerald Tuskan, and Daniel Jacobson

16. Connecting Microbial Genotype with Phenotype in the Omics Era

Yongfu Yang, Mengyu Qiu, Qing Yang, Yu Wang, Hui Wei, and Shihui Yang