Readily reproducible protocols for optimal histological observations under bright-field microscopy

Provides full description of plastic resin embedding methods for plant and animal research

Presents advanced methods to study leukocyte biology and inflammatory responses

Imaging techniques applied to cytogenetic, cancer, neurobiology, ion mobilization, and aquatic ecology studies

Includes immunocytochemistry methods and a comprehensive overview of fluorescence microscopy

Includes supplementary material: [...]

TABLE OF CONTENTS:

Table of Contents

Preface

Contributors

Part I. Bright-field Microscopy Applications

1. Glycol methacrylate embedding for improved morphological, morphometrical and immunohistochemical investigations under light microscopy: testes as a model
Hélio Chiarini-Garcia, Gleydes Gambogi Parreira and Fernanda Raddichi Almeida

2. Histological processing of teeth and periodontal tissues for light microscopy analysis.
Gerluza A. Borges Silva, Adriana Moreira and José Bento Alves

3. Large plant samples: how to process for 2-hydroxyethyl methacrylate embedding?
Élder Antônio Sousa Paiva, Sheila Zambello de Pinho and Denise Maria Trombert Oliveira

4. Image cytometry: nuclear and chromosomal DNA quantification
Carlos Roberto Carvalho, Wellington Ronildo Clarindo and Isabella Santiago de Abreu

5. Histological approaches to study tissue parasitism during Trypanosoma cruzi infection
Daniela L. Fabrino, Lívia Teixeira, Grazielle Ribeiro and Rossana C.N. Melo

6. Intravital microscopy to study leukocyte recruitment in vivo
Vanessa Pinho, Fernanda Matos Coelho, Gustavo Batista Menezes and Denise Carmona Cara

Part II. Fluorescence Microscopy Applications

7. Introduction to fluorescence microscopy
Ionita Ghiran

8. Using the fluorescent styryl dye FM1-43 to visualize synaptic vesicles exocytosis and
endocytosis in motor nerve terminals
Ernani Amaral, Silvia Guatimosim and Cristina Guatimosim

9. Imaging lipid bodies within leukocytes with different light microscopy techniques
Rossana C. N. Melo, Heloísa D¿Ávila, Patrícia T. Bozza and Peter F. Weller

10. EicosaCell ¿ An immunofluorescent-based assay to localize newly synthesized
eicosanoid lipid mediators at intracellular sites.
Christianne Bandeira-Melo, Peter F. Weller and Patricia T. Bozza

11. Nestin-driven green fluorescent protein as an imaging marker for nascent blood vessels in mouse models of cancer
Robert M. Hoffman

12. Imaging calcium sparks in cardiac myocytes
Silvia Guatimosim, Cristina Guatimosim and Long-Sheng Song

13. Light microscopy in aquatic ecology: methods for plankton communities studies
Maria Carolina S. Soares, Lúcia M. Lobão, Luciana O. Vidal, Natália P. Noyma, Nathan O. Barros, Fábio Roland

14. Fluorescence immunohistochemistry in combination with differential interference contrast microscopy for studies of semi-ultrathin specimens of epoxy resin-embedding samples.
Shin-ichi Iwasaki and Hidekazu Aoyagi