Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Restoration of podocyte phenotype in culture.- Protocols for the propagation, culture and characterization of kidney fibroblasts.- Isolation of rat glomeruli and propagation of mesangial cells to study the kidney in health and disease.- In vitro models to evaluate molecular permeability of the kidney filtration barrier.- Generating human glomeruli from pluripotent stem cells for disease modelling and drug screening.- Large-scale production of kidney organoids from human pluripotent stem cells.- Construction of a multitubular perfusable kidney-on-chip for the study of renal disease.- A "kidney-on-the-chip" model composed of primary human tubular, endothelial, and white blood cells.- Standardized protocol for the preparation of precision-cut kidney slices: a translational model of renal fibrosis.- Culture of three-dimensional Madin-Darby Canine Kidney (MDCK) cysts for in vitro drug testing in polycystic kidney disease.- Zebrafishmodel to study podocyte function within the glomerular filtration barrier.- Magnetic Resonance Imaging (MRI) analysis of tissue sodium concentration in chronic kidney disease.- Determination of interstitial collagen deposition and related topography using the second harmonic generation-based Histoindex platform.- Quantitative imaging of podocyte foot processes in the kidney using confocal and STED microscopy.- Synthesis and expression of a targeted, ferritin-based tracer for PET imaging of kidney glomeruli.- Multiplex in situ hybridization in the study of acute kidney injury.- Spatial transcriptomics in kidney tissue.- Assessment of kidney mitochondrial function by high-resolution respirometry, transmission electron microscopy and histological techniques.- Transdermal measurement of glomerular filtration rate in preclinical research.- The BioHybrid Assay - a novel method for determining calcification propensity.- Quantification of calciprotein particles (CPPs) in serum/plasma samples using a fluorescent bisphosphonate.- Monitoring of Rho GTPase Activity in Podocytes.