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Authoritative and cutting-edge, Ion Channels: Methods and Protocols, Second Edition will greatly assist researchers searching for specific methodology in studying ion channels on the path toward a greater understanding of these key biological features.
Authoritative and cutting-edge, Ion Channels: Methods and Protocols, Second Edition will greatly assist researchers searching for specific methodology in studying ion channels on the path toward a greater understanding of these key biological features.
Includes both well-established and cutting-edge methods and protocols that focus on ionic channel function
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: [...]
Approaches to Cloning of Pain-Related Ion Channel Genes.- Mammalian Expression Systems and Transfection Techniques.- Use of Escherichia coli for the Production and Purification of Membrane Proteins.- Transient Overexpression of Genes in Neurons Using Nucleofection.- Viral Gene Delivery: Optimized Protocol for Production of High Titre Lentiviral Vectors.- Two-Electrode Voltage Clamp.- Conventional Micropipette-Based Patch Clamp Techniques.- Recording of Ion Channel Activity in Planar Lipid Bilayers Experiments.- Recording Macroscopic Currents in Large Patches from Xenopus Oocytes.- Combined Single-Channel and Macroscopic Recording Techniques to Analyze Gating Mechanisms of the Large Conductance Ca2+ and Voltage Activated (BK) Potassium Channel.- Perforated Whole Cell Patch Clamp Recording.- Piezo-Electrically Driven Mechanical Stimulation of Sensory Neurons.- Automated Planar Patch-Clamp.- Recording Single-Channel Currents Using "Smart Patch-Clamp" Technique.- Using Total Internal Reflection Fluorescence Microscopy to Observe Ion channel Trafficking and Assembly.- Förster Resonance Energy Transfer-Based Imaging at the Cell Surface of Live Cells.- The Use of Dansyl-Calmodulin to Study Interactions with Channels and Other Proteins.- Imaging and Quantification of Recycled KATP Channels.- Generation of Antibodies that Are Externally-Acting Isoform-Specific Inhibitors of Ion Channels.- Site-Directed Mutagenesis to Study the Structure-Function Relationships of Ion Channels.- Cysteine-Based Cross-Linking Approach to Study Inter-Domain Interactions in Ion Channel Functions.- Analysis of Ca2+-Binding Sites in the MthK RCK Domain by X-RayCrystallography.- Isotope Labeling Strategies for Analysis of an Ion Channel Cytoplasmic Domain by NMR Spectroscopy.- Recording Dendritic Ion Channel Properties and Function from Cortical Neurons.- M Current Recording from Acute DRG Slices.- Studying Ion Channels in Human Erythrocytes by Direct and Indirect Means.- Recording Ion Channels in Isolated, Split-Opened Tubules.- Single Channel Analysis of TRPC Channels in the Podocytes of Freshly Isolated Glomeruli.- Ca2+ Imaging as a Tool to Assess TRP Channel Function in Murine Distal Nephrons.- Patch Clamping Drosophila Sensory Neurons.- Production and Validation of Recombinant Adeno-Associated Virus for Channelrhodopsin Expression in Neurons.- Optical Control of Ligand-Gated Ion Channels.
Erscheinungsjahr: | 2016 |
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Fachbereich: | Allgemeines |
Genre: | Biologie, Importe |
Rubrik: | Naturwissenschaften & Technik |
Thema: | Lexika |
Medium: | Taschenbuch |
Inhalt: |
xiv
440 S. |
ISBN-13: | 9781493960095 |
ISBN-10: | 1493960091 |
Sprache: | Englisch |
Einband: | Kartoniert / Broschiert |
Redaktion: | Gamper, Nikita |
Herausgeber: | Nikita Gamper |
Auflage: | Softcover reprint of the original 2nd edition 2013 |
Hersteller: | Humana Press |
Verantwortliche Person für die EU: | Humana Press in Springer Science + Business Media, Heidelberger Platz 3, D-14197 Berlin, juergen.hartmann@springer.com |
Maße: | 254 x 178 x 25 mm |
Von/Mit: | Nikita Gamper |
Erscheinungsdatum: | 23.08.2016 |
Gewicht: | 0,851 kg |