Michael E. Pfrender: Insect Genomics | Buch

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Cover: 9781493987740 | Insect Genomics | Methods and Protocols | Michael E. Pfrender (u. a.)

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Sprache: Englisch

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Beschreibung

This volume focuses on the latest methods used to sequence, assemble, and analyze insect genomes. The collection of protocols in this book provides an introduction to the workflows and bioinformatics tools available for researchers. The chapters cover a range of useful topics such as determining genome size by flow cytometry; High Molecular Weight DNA extraction; improvements to a genome assembly provided by long-range sequencing approaches; assessments of orthology and single-copy genes at different phylogenetic levels; detecting regulatory regions with FAIRE, RAMPAGE, and computational analysis of cis-regulatory modules in insects; bioinformatics analysis of epigenetic modifications, high-throughput scanning of insect genomes (TEEseq) for the presence of endosymbionts, and leveraging genome sequence information to design RNAi strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respectivetopics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Insect Genomics: Methods and Protocols is a valuable resource for graduate students, postdocs, and novice research scientists who are interested in learning more about this developing field.

Zusammenfassung

Includes cutting-edge techniques for the study of insect genomics

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts

Inhaltsverzeichnis

Arthropod Genome Sequencing and Assembly Strategies.- Genome Size Estimation and Quantitative Cytogenetics in Insects.- Isolation of HMW DNA from Insects Chapter.- Long range sequencing and assembly QC.- Integrated modeling of structural genes using MCuNovo.- Using BUSCO to Assess Insect Genomic Resources.- The GFF3toolkit-QC and Merge Pipeline for Genome Annotation.- Using Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE) to identify functional regulatory DNA in insect genomes.- Using RAMPAGE to identify and annotate promoters in insect genomes.- CRM Beyond Model Insects.- Whole-genome bisulfite sequencing for the methylation analysis of insect genomes.- Bioinformatic analysis of methylation patterns using bisulfite sequencing data.- Physical Genome Mapping Using Fluorescence in Situ Hybridization with Mosquito Chromosomes.- Target enriched RAD sequencing (TEEseq): A new high-throughput sequencing approach applied to the comprehensive characterization of endosymbionts.

Details

Erscheinungsjahr:	2018
Fachbereich:	Andere Fachgebiete
Genre:	Medizin
Rubrik:	Wissenschaften
Medium:	Buch
Seiten:	252
Reihe:	Methods in Molecular Biology
Inhalt:	xi 237 S. 11 s/w Illustr. 23 farbige Illustr. 237 p. 34 illus. 23 illus. in color.
ISBN-13:	9781493987740
ISBN-10:	1493987747
Sprache:	Englisch
Ausstattung / Beilage:	HC runder Rücken kaschiert
Einband:	Gebunden
Redaktion:	Pfrender, Michael E. Brown, Susan J.
Herausgeber:	Susan J Brown/Michael E Pfrender
Auflage:	1st ed. 2019
Hersteller:	Springer New York Springer US, New York, N.Y. Methods in Molecular Biology
Maße:	260 x 183 x 20 mm
Von/Mit:	Michael E. Pfrender (u. a.)
Erscheinungsdatum:	10.11.2018
Gewicht:	0,678 kg